Adekunle Abiodun Emmanuel, Wang Feng, Hu Jianhua, Ma Anzhou, Guo Chen, Zhuang Guoqiang, Liu Chun-Zhao
National Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
Bioprocess Biosyst Eng. 2015 Oct;38(10):1973-81. doi: 10.1007/s00449-015-1438-z. Epub 2015 Jul 16.
Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L(-1) chitosan to a 2-day-old culture media, with 24-h interval between the treatments. Under these conditions, laccase activity of 644.9 U l(-1) was achieved on the seventh day and laccase production was improved by 93.5 % higher than the control. Chitosan treatment increased reactive oxygen species generation and extracellular protein concentration in the treated mycelia. In contrast, the inducer inhibited the mycelia growth. The result of the quantitative reverse transcription polymerase chain reaction showed that the copy number of the laccase gene transcript increased by 16.7-fold in the treated mycelia relative to the control. This study provides insight into some of the intrinsic metabolic processes involved in the upregulation of laccase production in the presence of chitosan inducer in fungal culture.
开发了壳聚糖多次添加策略以提高云芝培养物中漆酶的产量。优化后的多次添加策略是在培养2天的培养基中分两次添加0.1 g L(-1)壳聚糖，两次处理间隔24小时。在这些条件下，第7天漆酶活性达到644.9 U l(-1)，漆酶产量比对照提高了93.5%。壳聚糖处理增加了处理过的菌丝体中活性氧的产生和细胞外蛋白质浓度。相反，诱导剂抑制了菌丝体生长。定量逆转录聚合酶链反应结果表明，处理过的菌丝体中漆酶基因转录本的拷贝数相对于对照增加了16.7倍。本研究深入了解了真菌培养中在壳聚糖诱导剂存在下漆酶产量上调所涉及的一些内在代谢过程。
