Allen Institute for Brain Science, Seattle, WA 98103, USA.
Instituto de Neurociencias UMH-CSIC, A03550 Alicante, Spain; Centro de Investigacion Biomedica en Red de Salud Mental (CIBERSAM) and IMIB-Arrixaca of Instituto de Salud Carlos III, 30120 Murcia, Spain.
Neuron. 2014 Jul 16;83(2):309-323. doi: 10.1016/j.neuron.2014.05.033. Epub 2014 Jun 19.
To provide a temporal framework for the genoarchitecture of brain development, we generated in situ hybridization data for embryonic and postnatal mouse brain at seven developmental stages for ∼2,100 genes, which were processed with an automated informatics pipeline and manually annotated. This resource comprises 434,946 images, seven reference atlases, an ontogenetic ontology, and tools to explore coexpression of genes across neurodevelopment. Gene sets coinciding with developmental phenomena were identified. A temporal shift in the principles governing the molecular organization of the brain was detected, with transient neuromeric, plate-based organization of the brain present at E11.5 and E13.5. Finally, these data provided a transcription factor code that discriminates brain structures and identifies the developmental age of a tissue, providing a foundation for eventual genetic manipulation or tracking of specific brain structures over development. The resource is available as the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org).
为了提供大脑发育的基因结构的时间框架，我们生成了大约 2100 个基因的胚胎和新生小鼠大脑的原位杂交数据，这些数据通过自动化信息学管道进行处理，并进行了手动注释。这个资源包括 434946 张图像、七个参考图谱、一个发生本体论和工具，用于探索基因在神经发育过程中的共表达。确定了与发育现象一致的基因集。检测到控制大脑分子组织的原则发生了时间上的转变，E11.5 和 E13.5 时大脑出现短暂的神经节、板状组织。最后，这些数据提供了一个转录因子代码，可以区分大脑结构，并确定组织的发育年龄，为最终的遗传操作或特定大脑结构在发育过程中的跟踪提供了基础。该资源可作为艾伦发育中的老鼠脑图谱（http://developingmouse.brain-map.org）获得。
