用于筛选植物来源的便捷、灵敏且高通量的方法。

Convenient, sensitive and high-throughput method for screening botanic origin.

作者信息

Yuan Yuan, Jiang Chao, Liu Libing, Yu Shulin, Cui Zhanhu, Chen Min, Lin Shufang, Wang Shu, Huang Luqi

机构信息

1] State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P. R. China [2].

1] Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, P. R. China [2].

出版信息

Sci Rep. 2014 Jun 23;4:5395. doi: 10.1038/srep05395.

DOI:10.1038/srep05395

PMID:24953704

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4066250/

Abstract

In this work, a rapid (within 4-5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products.

摘要

在这项工作中，通过将环介导等温扩增与阳离子共轭聚合物相结合，开发了一种快速（4-5小时内）、灵敏且可视化的评估植物来源的新方法。通过基因SNP基因分型分析，清晰地区分了形态和化学成分相似的两种中药材（金银花和山银花）。使用该检测系统成功地对含有忍冬属花蕾的专利中药中的植物种类进行了鉴定。该方法也足够稳健，可用于高通量筛选。这种新方法对鉴定草药材料非常有帮助，有利于检测植物产品的安全性和质量。