应用 DNA 条码鉴定五加科植物种。
Applying DNA barcodes for identification of plant species in the family Araliaceae.
机构信息
Center for Computational Biology and Bioinformatics, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
出版信息
Gene. 2012 May 10;499(1):76-80. doi: 10.1016/j.gene.2012.02.016. Epub 2012 Mar 2.
An effective DNA marker in authentication of the family Araliaceae was screened out of the five DNA regions (matK, rbcL, ITS2, psbA-trnH and ycf5). In the present study, 1113 sequences of 276 species from 23 genera (Araliaceae) were collected from DNA sequencing and GenBank, in which 16 specimens were from 5 provinces in China and Japan. All of the sequences were assessed in the success rates of PCR amplifications, intra- and inter-specific divergence, DNA barcoding gaps and efficiency of identification. Compared with other markers, ITS2 showed superiority in species discrimination with an accurate identification of 85.23% and 97.29% at the species and genus levels, respectively, in plant samples from the 589 sequences derived from Araliaceae. Consequently, as one of the most popular phylogenetic markers, our study indicated that ITS2 was a powerful barcode for Araliaceae identification.
从 5 个 DNA 区域（matK、rbcL、ITS2、psbA-trnH 和 ycf5）中筛选出一种鉴定五加科的有效 DNA 标记物。本研究从 DNA 测序和 GenBank 中收集了来自 23 个属（五加科）的 276 种 1113 个序列，其中 16 个标本来自中国和日本的 5 个省。所有序列的 PCR 扩增成功率、种内和种间分歧度、DNA 条形码间隙和鉴定效率均进行了评估。与其他标记物相比，ITS2 在物种鉴别方面具有优势，在 589 个五加科植物样本序列中，其在物种和属水平上的准确鉴定率分别为 85.23%和 97.29%。因此，作为最受欢迎的系统发育标记物之一，本研究表明 ITS2 是鉴定五加科的有效条形码。
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