Ernst Sabrina, Stübinger Stefan, Schüpbach Peter, Sidler Michéle, Klein Karina, Ferguson Stephen J, von Rechenberg Brigitte
Musculoskeletal Research Unit (MRSU), Vetsuisse Faculty ZH, Equine Hospital, University of Zurich, Zurich, Switzerland.
Center for Applied Biotechnology and Molecular Medicine (CABMM), Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Clin Oral Implants Res. 2015 Aug;26(8):898-908. doi: 10.1111/clr.12411. Epub 2014 May 21.
The aim of this study was to compare two different surfaces of one uniform macro-implant design in order to focus exclusively on the osseointegration properties after 2, 4 and 8 weeks and to discuss the animal model chosen.
In six mature sheep, n = 36 implants with a highly crystalline and phosphate-enriched anodized titanium oxide surface (TiU) and n = 36 implants with a hydrophilic, sandblasted, large grit and acid-etched surface (SLA) were placed in the pelvic bone. TiU implants were custom-made to match the SLA implant design. The implant stability and bone-to-implant contact (BIC) were assessed by resonance frequency (ISQ), backscatter scanning electron microscopy (B-SEM), light microscopy (LM), micro-CT and intravital fluorochrome staining. Biomechanical removal torque testing was performed.
Overall, no statistically significant differences in BIC total (trabecular + cortical) between TiU and SLA were found via LM and B-SEM. BIC values (B-SEM; LM) in both groups revealed a steady rise in trabecular bone attachment to the implant surface after 2, 4 and 8 weeks. In the 2- to 4-week time interval in the TiU group (P = 0.005) as well as in the SLA group (P = 0.01), a statistically significant increase in BIC trabecular could be observed via LM. B-SEM values confirmed the statistically significant increase for TiU (P = 0.001). In both groups, BIC trabecular values after 8 weeks were significantly higher (P ≤ 0.05) than after 2 weeks (B-SEM; LM). Biomechanical data confirmed the histological data.
The two surfaces proved comparable osseointegration in this sheep model.
本研究旨在比较一种统一的宏观种植体设计的两种不同表面，以便专门关注2周、4周和8周后的骨整合特性，并讨论所选用的动物模型。
在6只成年绵羊的骨盆骨中植入36枚具有高度结晶且富含磷酸盐的阳极氧化钛表面（TiU）的种植体和36枚具有亲水性、喷砂、大颗粒和酸蚀表面（SLA）的种植体。TiU种植体是定制的，以匹配SLA种植体设计。通过共振频率（ISQ）、背散射扫描电子显微镜（B-SEM）、光学显微镜（LM）、显微CT和活体荧光染料染色评估种植体稳定性和骨与种植体接触（BIC）。进行了生物力学去除扭矩测试。
总体而言，通过LM和B-SEM发现TiU和SLA之间的BIC总量（小梁骨+皮质骨）无统计学显著差异。两组的BIC值（B-SEM；LM）显示，2周、4周和8周后小梁骨与种植体表面的附着稳步增加。在TiU组（P = 0.005）以及SLA组（P = 0.01）的2至4周时间间隔内，通过LM可观察到BIC小梁骨有统计学显著增加。B-SEM值证实了TiU的统计学显著增加（P = 0.001）。两组中，8周后的BIC小梁骨值均显著高于2周后（B-SEM；LM）（P≤0.05）。生物力学数据证实了组织学数据。
在该绵羊模型中，这两种表面的骨整合效果相当。
