Institute of Materials, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Musculoskeletal Research Unit, University of Zürich, Zürich, Switzerland.
Nat Commun. 2019 May 2;10(1):2062. doi: 10.1038/s41467-019-09673-1.
The prediction of implant behavior in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. Simulated body fluids (SBFs) have been proposed and widely used to evaluate the bone-bonding ability of implant materials. In view of its limitations, we report here a rapid in vitro method based on calcium titration for the evaluation of in vivo bioactivity. Using four different titanium surfaces, this method identifies that alkaline treatment is the key process to confer bioactivity to titanium whereas no significant effect from heat treatment is observed. The presence of bioactive titanium surfaces in the solution during calcium titration induces an earlier nucleation of crystalline calcium phosphates and changes the crystallization pathway. The conclusions from this method are also supported by the standard SBF test (ISO 23317), in vitro cell culture tests using osteoblasts and in vivo animal experiments employing a pelvic sheep model.
在生物材料研究中，通过易于实施的体外方法来预测植入物的体内行为具有重要意义。模拟体液（SBF）已被提出并广泛用于评估植入材料的骨结合能力。鉴于其局限性，我们在此报告了一种基于钙滴定的快速体外方法，用于评估体内生物活性。使用四种不同的钛表面，该方法表明碱性处理是赋予钛生物活性的关键过程，而热处理则没有明显效果。钙滴定过程中溶液中存在具有生物活性的钛表面会导致结晶性磷酸钙更早地成核，并改变结晶途径。该方法的结论还得到了标准 SBF 测试（ISO 23317）、使用成骨细胞的体外细胞培养测试以及使用骨盆绵羊模型的体内动物实验的支持。
