Rokicki Wojciech, Żaba Małgorzata, Mrukwa-Kominek Ewa, Wyględowska-Promieńska Dorota, Brzozowa Marlena, Reichman-Warmusz Edyta, Wojnicz Romuald
Department and Clinic of Ophthalmology, Medical University of Silesia, Katowice, Poland.
Department of Histology and Embryology, Medical University of Silesia, Zabrze, Poland.
Med Sci Monit. 2014 Jun 23;20:1051-5. doi: 10.12659/MSM.890176.
Previous reports have indicated the role of endothelium disturbances, as expressed by von Willebrand factor (vWF) release, in pathophysiology of glaucoma. The objective of this study was to investigate the vWF expression in iris vasculature of patients with primary open-angle glaucoma (POAG).
Immunohistochemistry of vWF expression was performed on cryostat sections of samples collected at the time of peripheral iridectomy and controls collected from dead donors.
Twenty-seven Caucasians age 66.6±3.7 with 5.8±3.7-year history of treated PAOG and 10 controls age 62.2±1.92 with no history of glaucoma. The percentage of patients who presented normal and up-regulation of vWF phenotype expression differed statistically between examined and control groups: 48% versus 100% (p=0.035, chi-square test with Yates' correction). Sex, age, glaucoma duration, and visual field quantitative indices had no impact on vWF expression. A significant correlation between mean pre-surgery intraocular pressure and vWF expression was found (Spearman r=0.42, p=0.03).
Considering the results, it may be suggested that vWF is actively involved in the pathophysiology of glaucoma.
既往报告表明，以血管性血友病因子（vWF）释放为表现的内皮功能紊乱在青光眼病理生理学中发挥作用。本研究的目的是调查原发性开角型青光眼（POAG）患者虹膜血管中vWF的表达情况。
对周边虹膜切除术时采集的样本冰冻切片以及从死亡供体采集的对照样本进行vWF表达的免疫组织化学检测。
27例白种人，年龄66.6±3.7岁，有5.8±3.7年治疗PAOG病史；10例对照，年龄62.2±1.92岁，无青光眼病史。vWF表型表达正常和上调的患者百分比在检查组和对照组之间存在统计学差异：48% 对100%（p = 0.035，采用Yates校正的卡方检验）。性别、年龄、青光眼病程和视野定量指标对vWF表达无影响。发现术前平均眼压与vWF表达之间存在显著相关性（Spearman r = 0.42，p = 0.03）。
综合研究结果，提示vWF可能积极参与青光眼的病理生理过程。
