Rokicki Wojciech, Żaba Małgorzata, Wyględowska-Promieńska Dorota, Kabiesz Adam, Reichman-Warmusz Edyta, Brzozowa Marlena, Majewski Wojciech, Wojnicz Romuald
Ophthalmology Clinic and Department of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
Department of Histology & Embryology, School of Medicine in Katowice, Katowice, Poland.
Med Sci Monit. 2015 Jan 7;21:76-81. doi: 10.12659/MSM.891177.
The "double-faced" effect of nitric oxide (NO) is thought to play an important role in triggering and progression of glaucoma.
MATERIAL/METHODS: Iris samples were obtained during iridectomy in 35 patients (mean age of 65.4±5.3 years) with diagnosed primary open-angle glaucoma (POAG). The controls were collected postmortem from 10 donors with a mean age of 62.2±1.9 years. Visual field defects were evaluated by perimetry. The Hodapp-Parrish-Anderson classification was used to divide patients into 3 visual field defect groups. The intraocular pressure was measured 3 times before surgery using applanation tonometry. The phenotype activity of nitric oxide synthase (NOS) isoenzymes (endothelial--eNOS and inducible--iNOS) and expression of nitrotyrosine in iris vasculature was assessed.
Significant differences were found between glaucoma patients and the controls in eNOS and iNOS activity (Mann-Whitney test, U=35.5, Z=-2.037, p=0.04 and U=21, Z=2.69, p=0.007, respectively). In addition, the results showed an upregulation of nitrotyrosine in the capillary endothelial cells in the study group, which was associated with the duration of diagnosed glaucoma (R-Spearman of 0.33, p=0.0047) and visual field mean defect MD (R-Spearman of 0.29, p=0.019). Moreover, the activity of nitrotyrosine was significantly correlated with iNOS immunoreactivity (R-Spearman of 0.5, p=0.0001). However, the iNOS activity significantly varied among Hodapp-Parrish-Anderson groups (p=0.03).
Our observations confirmed the association between glaucomatous disturbances and upregulation of iNOS, together with increased nitrotyrosine storage.
一氧化氮（NO）的“双面”效应被认为在青光眼的引发和进展中起重要作用。
材料/方法：在35例（平均年龄65.4±5.3岁）诊断为原发性开角型青光眼（POAG）的患者进行虹膜切除术时获取虹膜样本。对照组为10例平均年龄62.2±1.9岁的死后捐献者的样本。通过视野计评估视野缺损情况。采用霍达普 - 帕里什 - 安德森分类法将患者分为3个视野缺损组。术前使用压平眼压计测量眼压3次。评估一氧化氮合酶（NOS）同工酶（内皮型 - eNOS和诱导型 - iNOS）的表型活性以及虹膜血管中硝基酪氨酸的表达。
青光眼患者与对照组在eNOS和iNOS活性方面存在显著差异（曼 - 惠特尼检验，U = 35.5，Z = -2.037，p = 0.04；U = 21，Z = 2.69，p = 0.007）。此外，结果显示研究组毛细血管内皮细胞中硝基酪氨酸上调，这与青光眼诊断时长（斯皮尔曼相关系数R = 0.33，p = 0.0047）和视野平均缺损MD（斯皮尔曼相关系数R = 0.29，p = 0.019）相关。而且，硝基酪氨酸活性与iNOS免疫反应性显著相关（斯皮尔曼相关系数R = 0.5，p = 0.0001）。然而，iNOS活性在霍达普 - 帕里什 - 安德森组间有显著差异（p = 0.03）。
我们的观察结果证实了青光眼病变与iNOS上调以及硝基酪氨酸储存增加之间的关联。
