共济失调蛋白-3的表达与胃癌的临床病理特征相关。
Ataxin-3 expression correlates with the clinicopathologic features of gastric cancer.
作者信息
Zeng Li-Xia, Tang Yong, Ma Yun
机构信息
Department of Pathology, The First Affiliate Hospital of Guangxi Medical University No. 21, Shuangyong RD, Nanning 530021, China.
Departments of Urology Surgery, Cancer Hospital of Guangxi Medical University No. 71, Nanning 530021, China.
出版信息
Int J Clin Exp Med. 2014 Apr 15;7(4):973-81. eCollection 2014.
UNLABELLED
To investigate the expression of Ataxin-3 in human gastric cancer tissues and cell lines, and explore its clinical pathologic significance.
METHODS
The expression of Ataxin-3 in gastric cancer (n=536) and noncancerous gastric mucosa (n=312) was determined by immunohistochemistry and correlated to clinicopathologic features such as histologic differentiation and tumor size. The expression of Ataxin-3 protein in the human gastric cancer cell lines MKN45, SGC7901 and in normal human gastric epithelial cells (GES-1) was also evaluated by Western blot analysis. Quantitative real-time PCR was used to determine Ataxin-3 mRNA expression in human gastric cancer cell lines and tissues.
RESULTS
The expression of Ataxin-3 protein was decreased in the gastric cancer compared to noncancerous gastric tissue, and correlated with tumor size, Lauren classification, histologic differentiation, and mutant p53 protein (P < 0.05). Similarly, Ataxin-3 mRNA expression was decreased in the gastric cancers compared to the noncancerous gastric tissue. Ataxin-3 protein and mRNA expression was lower in MKN45, SGC7901 cells than in the normal GES-1 cells.
CONCLUSION
Decreased expression of Ataxin-3 may play an important role in gastric carcinogenesis and development of gastric cancer.
未标记
研究Ataxin-3在人胃癌组织和细胞系中的表达，并探讨其临床病理意义。
方法
采用免疫组织化学法检测Ataxin-3在胃癌组织（n = 536）和非癌胃黏膜组织（n = 312）中的表达，并与组织学分化、肿瘤大小等临床病理特征进行相关性分析。采用蛋白质印迹法检测Ataxin-3蛋白在人胃癌细胞系MKN45、SGC7901及正常人胃上皮细胞（GES-1）中的表达。采用定量实时聚合酶链反应检测Ataxin-3 mRNA在人胃癌细胞系和组织中的表达。
结果
与非癌胃组织相比，胃癌组织中Ataxin-3蛋白表达降低，且与肿瘤大小、Lauren分类、组织学分化及突变型p53蛋白相关（P < 0.05）。同样，与非癌胃组织相比，胃癌组织中Ataxin-3 mRNA表达降低。MKN45、SGC7901细胞中Ataxin-3蛋白和mRNA表达低于正常GES-1细胞。
结论
Ataxin-3表达降低可能在胃癌发生发展中起重要作用。
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