Ek Rauf Onur, Serter Mukadder, Ergin Kemal, Cecen Serpil, Unsal Cengiz, Yildiz Yuksel, Bilgin Mehmet D
Department of Physiology, Adnan Menderes University Aydin, Turkey.
Department of Biochemistry, Adnan Menderes University Aydin, Turkey.
Int J Clin Exp Med. 2014 Apr 15;7(4):989-97. eCollection 2014.
The aim of this study was to investigate the effects of citicoline on the development of colitis and antioxidant parameters in rats subjected to tribenzene sulfonic acid (TNBS)-induced colitis. Twenty four Wistar Albino female rats were divided into four subgroups (n=6) (control, colitis control, colitis + 50 mg/kg citicoline, colitis + 250 mg/kg citicoline). Colitis was induced using an enema of TNBS and ethanol; following which citicoline was administrated for 3 days and effects of citicoline was subsequently evaluated. Based on microscopic damage scores, there was no difference between rats of the TNBS-colitis and 50 mg/kg citicoline treated groups, whereas treatment with 250 mg/kg citicoline, caused significant reduction in colon injury compared to that observed in rats of TNBS-colitis group. In terms of the biochemical analyses, myeloperoxidase (MPO), malondialdehyde (MDA), reduced glutathione (GSH), and IL-6 levels in rats from 250 mg/kg citicoline group were significantly different from that TNBS-colitis group. The levels of MPO, MDA, GSH and IL-6 in control rats were also significantly different those of rats in the TNBS-colitis group. Citicoline may have a positive protective effect on the inflammatory bowel disease treatment process and could, therefore, be used as an adjunct therapy in colitis. These effects of citicoline may exist through anti-inflammatory and antioxidant mechanism.
本研究旨在探讨胞磷胆碱对三硝基苯磺酸（TNBS）诱导的大鼠结肠炎发展及抗氧化参数的影响。将24只Wistar白化雌性大鼠分为四个亚组（n = 6）（对照组、结肠炎对照组、结肠炎 + 50 mg/kg胞磷胆碱组、结肠炎 + 250 mg/kg胞磷胆碱组）。使用TNBS和乙醇灌肠诱导结肠炎；随后给予胞磷胆碱3天，并评估胞磷胆碱的效果。根据显微镜损伤评分，TNBS结肠炎组和50 mg/kg胞磷胆碱治疗组的大鼠之间没有差异，而与TNBS结肠炎组大鼠相比，250 mg/kg胞磷胆碱治疗导致结肠损伤显著减轻。在生化分析方面，250 mg/kg胞磷胆碱组大鼠的髓过氧化物酶（MPO）、丙二醛（MDA）、还原型谷胱甘肽（GSH）和白细胞介素-6水平与TNBS结肠炎组有显著差异。对照组大鼠的MPO、MDA、GSH和IL-6水平与TNBS结肠炎组大鼠也有显著差异。胞磷胆碱可能对炎症性肠病治疗过程具有积极的保护作用，因此可作为结肠炎的辅助治疗药物。胞磷胆碱的这些作用可能通过抗炎和抗氧化机制存在。
