Feyzioglu Bahadir, Dogan Metin, Sanli Ozlem O, Ozdemir Mehmet, Baykan Mahmut
Department of Medical Microbiology, Meram Medicine of Faculty, Necmettin Erbakan University Konya, Turkey.
Int J Clin Exp Med. 2014 Apr 15;7(4):1084-8. eCollection 2014.
Tuberculosis is a common infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis (TB). Various liquid or solid media are used for the diagnosis of tuberculosis. TK Rapid Mycobacterial Culture System has been developed recently. In our study, we aimed to compare TK Rapid Mycobacterial Culture System with LJ and MGIT systems in the diagnosis of tuberculosis. 200 clinical specimens (152 sputum, 41 Bronchoalveolar lavage fluid (BAL), 4 gastric aspirations, 2 urine and 1 wound) obtained from 192 patients from different clinics were included for the diagnosis of TB. All specimens were decontaminated by using the same-common procedure in all the methods. The obtained sediment was used for inoculation for the BACTEC MGIT 960, TK and LJ. Additionally, smears were prepared from the residual suspension for Ehrlich-Ziehl-Neelsen (EZN) staining for microscopic examination. Contamination was observed in 23 sputum and 4 BAL samples. Contamination rates for TK, LJ, and BACTEC MGIT 960 systems were determined as 3 (1.5%), 13 (6.5%), and 18 (9%) respectively. Mycobacterium tuberculosis growth was determined as 15 (7.5%), 14 (7%) and 13 (6.5%) by TK culture system, MGIT and LJ, respectively. In our study, the total mean detection times of Mycobacterium tuberculosis by the LJ, TK, and MGIT method were 20.1, 17.1, and 8.3 days, respectively. TK system showed a dramatically lower contamination rate than the others. There was no difference in growth rates for each of the three methods. We concluded that the TK culture system is disadvantageous in terms of turnaround time.
结核病是一种由多种分枝杆菌菌株引起的常见传染病，通常由结核分枝杆菌（TB）引起。各种液体或固体培养基用于结核病的诊断。TK快速分枝杆菌培养系统是最近开发的。在我们的研究中，我们旨在比较TK快速分枝杆菌培养系统与LJ和MGIT系统在结核病诊断中的效果。纳入了从不同诊所的192名患者获得的200份临床标本（152份痰液、41份支气管肺泡灌洗液（BAL）、4份胃抽吸物、2份尿液和1份伤口标本）用于结核病诊断。所有标本均采用所有方法中相同的常规程序进行净化处理。获得的沉淀物用于接种BACTEC MGIT 960、TK和LJ。此外，从剩余悬液中制备涂片进行埃利希-齐尔-尼尔森（EZN）染色以进行显微镜检查。在23份痰液和4份BAL样本中观察到污染。TK、LJ和BACTEC MGIT 960系统的污染率分别确定为3（1.5%）、13（6.5%）和18（9%）。通过TK培养系统、MGIT和LJ分别确定结核分枝杆菌生长率为15（7.5%）、14（7%）和13（6.5%）。在我们的研究中，LJ、TK和MGIT方法检测结核分枝杆菌的总平均检测时间分别为20.1天、17.1天和8.3天。TK系统的污染率明显低于其他系统。三种方法各自的生长率没有差异。我们得出结论，TK培养系统在周转时间方面存在劣势。
