使用自动BACTEC MGIT 960系统、BACTEC 460 TB系统和罗-琴培养基对结核分枝杆菌回收率的比较。
Comparison of recoveries of mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Löwenstein-Jensen medium.
作者信息
Somoskövi A, Ködmön C, Lantos A, Bártfai Z, Tamási L, Füzy J, Magyar P
机构信息
Department of Respiratory Medicine, Semmelweis University, H-1536 Budapest 114, Hungary.
出版信息
J Clin Microbiol. 2000 Jun;38(6):2395-7. doi: 10.1128/JCM.38.6.2395-2397.2000.
Abstract
Using two different liquid media and one conventional solid medium, a total of 57 mycobacterial isolates (Mycobacterium tuberculosis, n = 55; nontuberculous mycobacteria, n = 2) were recovered from 377 clinical specimens. The rates of recovery of M. tuberculosis were 96. 4% with the BACTEC MGIT 960 liquid medium, 92.7% with BACTEC 12B liquid medium, and 81.8% with the Löwenstein-Jensen (LJ) medium. The mean time to detection of M. tuberculosis in smear-positive specimens was 12.6 days for BACTEC MGIT 960 medium, 13.8 days for BACTEC 12B medium, and 20.1 days for LJ medium, and in smear-negative specimens it was 15.8 days for BACTEC MGIT 960 medium, 17.7 days for BACTEC 12B medium, and 42.2 days for LJ medium. The rates of contamination were 3.7, 2.9, and 1.2% for the BACTEC MGIT 960, BACTEC 12B, and LJ media, respectively. In conclusion, the nonradiometric, fully automated 7-ml BACTEC MGIT 960 system can be considered a viable alternative to the semiautomated, radiometric BACTEC 460 TB system.
摘要
使用两种不同的液体培养基和一种传统固体培养基，从377份临床标本中共分离出57株分枝杆菌（结核分枝杆菌，n = 55；非结核分枝杆菌，n = 2）。结核分枝杆菌在BACTEC MGIT 960液体培养基中的回收率为96.4%，在BACTEC 12B液体培养基中的回收率为92.7%，在罗-琴（LJ）培养基中的回收率为81.8%。涂片阳性标本中结核分枝杆菌的平均检测时间，BACTEC MGIT 960培养基为12.6天，BACTEC 12B培养基为13.8天，LJ培养基为20.1天；涂片阴性标本中，BACTEC MGIT 960培养基为15.8天，BACTEC 12B培养基为17.7天，LJ培养基为42.2天。BACTEC MGIT 960、BACTEC 12B和LJ培养基的污染率分别为3.7%、2.9%和1.2%。总之，非放射性、全自动的7毫升BACTEC MGIT 960系统可被视为半自动放射性BACTEC 460 TB系统的可行替代方案。
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