Zhang Xue-De, Li Wei, Zhang Na, Hou Yan-Li, Niu Ze-Qun, Zhong Yu-Jie, Zhang Yu-Ping, Yang Shuan-Ying
Department of Respiratory Medicine, The Second Affiliated Hospital, Xi'an Jiaotong University 157 Xi 5 Road, Xi'an, Shannxi, China.
Int J Clin Exp Med. 2014 Apr 15;7(4):1190-6. eCollection 2014.
In our previous study, the upregulation of adipophilin in lung adenocarcinoma were identified compared with normal lung tissues by quantitative proteomics. In this study, our aim was to verify the result from quantitative proteomics, further investigate the relationship between adipophilin expression and clinicopathologic factors of lung cancer patients. The expression levels of adipophilin were examined in 10 pairs of lung adenocarcinoma and normal lung tissues using western blotting and the expression and cellular distribution of adipophilin were determined by IHC in 62 formalin-fixed and paraffin embedded primary lung cancer specimens. Adipophilin expression was significantly higher in lung adenocarcinoma specimens than in normal tissues and lung squamous cell carcinomas (P<0.05). There were no significant difference of adipophilin expression between lung squamous cell carcinomas and normal lung tissues. The expression of adipophilin in lung cancer did not correlate with any clinicopathologic factors such as lymph node metastasis, patients' age, gender, tumor size, grade, and TNM stage. In Conclusion, Adipophilin was upregulated in lung adenocarcinoma, suggesting that adipophilin play an important role in tumorigenesis of lung adenocarcinoma and may serve as a potential marker for lung adenocarcinoma.
在我们之前的研究中，通过定量蛋白质组学发现，与正常肺组织相比，肺腺癌中脂联素上调。在本研究中，我们的目的是验证定量蛋白质组学的结果，进一步研究脂联素表达与肺癌患者临床病理因素之间的关系。使用蛋白质免疫印迹法检测了10对肺腺癌组织和正常肺组织中脂联素的表达水平，并通过免疫组化法在62例福尔马林固定石蜡包埋的原发性肺癌标本中测定了脂联素的表达及细胞分布。肺腺癌标本中脂联素的表达明显高于正常组织和肺鳞癌（P<0.05）。肺鳞癌与正常肺组织中脂联素的表达无显著差异。肺癌中脂联素的表达与任何临床病理因素，如淋巴结转移、患者年龄、性别、肿瘤大小、分级和TNM分期均无相关性。总之，脂联素在肺腺癌中上调，提示脂联素在肺腺癌的肿瘤发生中起重要作用，可能作为肺腺癌的潜在标志物。
