Curcio Manuela, Cirillo Giuseppe, Parisi Ortensia Ilaria, Iemma Francesca, Picci Nevio, Puoci Francesco
Department of Pharmaceutical Sciences, University of Calabria, Arcavacata di Rende (CS) 87036, Italy.
J Funct Biomater. 2012 Mar 29;3(2):269-82. doi: 10.3390/jfb3020269.
In this work, molecularly imprinted nanospheres for controlled/sustained release of quercetin were synthesized employing methacrylic acid and ethylene glycoldymethacrylate as functional monomer and crosslinking agent, respectively. One pot precipitation polymerization was chosen as polymerization technique to obtain nanosized materials with spherical shape. Morphological and hydrophilic properties by scanning electron microscopy and water content measurements were determined, and recognition and selectivity properties of the imprinted materials were tested using the template quercetin and its structural analogue, the flavonoid catechin. Finally, the applicability of the obtained materials as drug delivery devices was evaluated by performing in vitro release studies in plasma simulating fluids and cytotoxicity testson HeLa cells.
在本研究中，分别以甲基丙烯酸和乙二醇二甲基丙烯酸酯作为功能单体和交联剂，合成了用于槲皮素控释/缓释的分子印迹纳米球。采用一锅沉淀聚合法作为聚合技术来制备具有球形的纳米材料。通过扫描电子显微镜和水分含量测量确定了材料的形态和亲水性能，并使用模板槲皮素及其结构类似物黄酮类儿茶素测试了印迹材料的识别和选择性性能。最后，通过在血浆模拟液中进行体外释放研究以及对HeLa细胞进行细胞毒性测试，评估了所得材料作为药物递送装置的适用性。
