Vashisth Priya, Kumar Naresh, Sharma Mohit, Pruthi Vikas
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Biotechnol Rep (Amst). 2015 Sep 14;8:36-44. doi: 10.1016/j.btre.2015.08.008. eCollection 2015 Dec.
Ferulic acid is a ubiquitous phytochemical that holds enormous therapeutic potential but has not gained much consideration in biomedical sector due to its less bioavailability, poor aqueous solubility and physiochemical instability. In present investigation, the shortcomings associated with agro-waste derived ferulic acid were addressed by encapsulating it in electrospun nanofibrous matrix of poly (d,l-lactide-co-glycolide)/polyethylene oxide. Fluorescent microscopic analysis revealed that ferulic acid predominantly resides in the core of PLGA/PEO nanofibers. The average diameters of the PLGA/PEO and ferulic acid encapsulated PLGA/PEO nanofibers were recorded as 125 ± 65.5 nm and 150 ± 79.0 nm, respectively. The physiochemical properties of fabricated nanofibers are elucidated by IR, DSC and NMR studies. Free radical scavenging activity of fabricated nanofibers were estimated using di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed the cytotoxicity of ferulic acid encapsulated nanofibers against hepatocellular carcinoma (HepG2) cells. These ferulic acid encapsulated nanofibers could be potentially explored for therapeutic usage in biomedical sector.
阿魏酸是一种普遍存在的植物化学物质，具有巨大的治疗潜力，但由于其生物利用度较低、水溶性差和理化性质不稳定，在生物医学领域尚未得到太多关注。在本研究中，通过将阿魏酸封装在聚（d，l-丙交酯-共-乙交酯）/聚环氧乙烷的电纺纳米纤维基质中，解决了与农业废弃物衍生的阿魏酸相关的缺点。荧光显微镜分析表明，阿魏酸主要存在于聚乳酸-乙醇酸共聚物/聚环氧乙烷纳米纤维的核心部位。聚乳酸-乙醇酸共聚物/聚环氧乙烷纳米纤维和阿魏酸封装的聚乳酸-乙醇酸共聚物/聚环氧乙烷纳米纤维的平均直径分别记录为125±65.5纳米和150±79.0纳米。通过红外光谱（IR）、差示扫描量热法（DSC）和核磁共振（NMR）研究阐明了制备的纳米纤维的理化性质。使用二（苯基）-（2,4,6-三硝基苯基）亚氨基氮鎓（DPPH）测定法评估制备的纳米纤维的自由基清除活性。3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）测定法证实了阿魏酸封装的纳米纤维对肝癌（HepG2）细胞的细胞毒性。这些阿魏酸封装的纳米纤维在生物医学领域具有潜在的治疗用途。
