Han Ning, Johnson Jed K, Bradley Patrick A, Parikh Kunal S, Lannutti John J, Winter Jessica O
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA.
Department of Materials and Science Engineering, The Ohio State University, Columbus, OH 43210, USA.
J Funct Biomater. 2012 Jul 27;3(3):497-513. doi: 10.3390/jfb3030497.
Hydrogels, electrospun fiber mats (EFMs), and their composites have been extensively studied for tissue engineering because of their physical and chemical similarity to native biological systems. However, while chemically similar, hydrogels and electrospun fiber mats display very different topographical features. Here, we examine the influence of surface topography and composition of hydrogels, EFMs, and hydrogel-EFM composites on cell behavior. Materials studied were composed of synthetic poly(ethylene glycol) (PEG) and poly(ethylene glycol)-poly(ε-caprolactone) (PEGPCL) hydrogels and electrospun poly(caprolactone) (PCL) and core/shell PCL/PEGPCL constituent materials. The number of adherent cells and cell circularity were most strongly influenced by the fibrous nature of materials (e.g., topography), whereas cell spreading was more strongly influenced by material composition (e.g., chemistry). These results suggest that cell attachment and proliferation to hydrogel-EFM composites can be tuned by varying these properties to provide important insights for the future design of such composite materials.
水凝胶、电纺纤维垫（EFM）及其复合材料因其与天然生物系统在物理和化学性质上的相似性，已被广泛用于组织工程研究。然而，尽管水凝胶和电纺纤维垫在化学性质上相似，但它们呈现出非常不同的拓扑特征。在此，我们研究了水凝胶、EFM以及水凝胶-EFM复合材料的表面拓扑结构和组成对细胞行为的影响。所研究的材料由合成聚乙二醇（PEG）和聚乙二醇-聚己内酯（PEGPCL）水凝胶、电纺聚己内酯（PCL）以及核/壳PCL/PEGPCL组成材料构成。贴壁细胞数量和细胞圆形度受材料的纤维性质（如拓扑结构）影响最大，而细胞铺展则受材料组成（如化学性质）影响更大。这些结果表明，通过改变这些特性可以调节细胞对水凝胶-EFM复合材料的附着和增殖，为这类复合材料的未来设计提供重要见解。
