Horvathova Eva, Navarova Jana, Galova Eliska, Sevcovicova Andrea, Chodakova Lenka, Snahnicanova Zuzana, Melusova Martina, Kozics Katarina, Slamenova Darina
Department of Genetics, Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 91 Bratislava, Slovak Republic.
J Agric Food Chem. 2014 Jul 16;62(28):6632-9. doi: 10.1021/jf501006y. Epub 2014 Jul 2.
Selected components of plant essential oils and intact Rosmarinus officinalis oil (RO) were investigated for their antioxidant, iron-chelating, and DNA-protective effects. Antioxidant activities were assessed using four different techniques. DNA-protective effects on human hepatoma HepG2 cells and plasmid DNA were evaluated with the help of the comet assay and the DNA topology test, respectively. It was observed that whereas eugenol, carvacrol, and thymol showed high antioxidative effectiveness in all assays used, RO manifested only antiradical effect and borneol and eucalyptol did not express antioxidant activity at all. DNA-protective ability against hydrogen peroxide (H2O2)-induced DNA lesions was manifested by two antioxidants (carvacrol and thymol) and two compounds that do not show antioxidant effects (RO and borneol). Borneol was able to preserve not only DNA of HepG2 cells but also plasmid DNA against Fe(2+)-induced damage. This paper evaluates the results in the light of experiences of other scientists.
对植物精油的选定成分以及完整的迷迭香油（RO）进行了抗氧化、铁螯合和DNA保护作用的研究。使用四种不同技术评估抗氧化活性。分别借助彗星试验和DNA拓扑测试评估对人肝癌HepG2细胞和质粒DNA的DNA保护作用。结果发现，丁香酚、香芹酚和百里香酚在所有使用的试验中均表现出高抗氧化效力，而RO仅表现出抗自由基作用，冰片和桉叶油根本不表现出抗氧化活性。两种抗氧化剂（香芹酚和百里香酚）以及两种不显示抗氧化作用的化合物（RO和冰片）表现出对过氧化氢（H2O2）诱导的DNA损伤的DNA保护能力。冰片不仅能够保护HepG2细胞的DNA，还能保护质粒DNA免受Fe(2+)诱导的损伤。本文根据其他科学家的经验对结果进行了评估。
