Slamenová D, Horváthová E, Sramková M, Marsálková L
Cancer Research Institute of Slovak Academy of Science, Bratislava, Slovakia.
Neoplasma. 2007;54(2):108-12.
Many components of essential volatile oils show antioxidant activity and may serve e.g. as a natural replacement of synthetic antioxidant food additives. However, it is important to evaluate such compounds also for their pro-oxidant and toxic properties as their plant origin doesn't secure their safety for living beings, including humans. The aim of this study was therefore to investigate cytotoxic, genotoxic and DNA-protective effects of the long-term (24 h) incubation of mammalian cells with two components of essential plant oils (carvacrol and thymol) in in vitro conditions. Cytotoxicity testing was in all cell lines (human hepatoma cells HepG2, human colonic cells Caco-2 and hamster lung cells V79) performed on the basis of trypan blue exclusion. Plating efficiency was evaluated only in V79 cells which manifest a high colony forming ability. The amount of DNA lesions induced in cells treated with hydrogen peroxide, carvacrol, thymol or combinations of carvacrol or thymol with hydrogen peroxide was measured by standard alkaline single cell gel electrophoresis in human cells HepG2 and Caco-2. Trypan blue exclusion test showed that carvacrol was mildly more cytotoxic than thymol and that Caco-2 cells were mildly more resistant to both carvacrol and thymol than HepG2 and V79 cells. At concentrations = IC20-40, the compounds studied did not induce DNA strand breaks either in human cells HepG2 or in cells Caco-2. Incubation of HepG2 and Caco- 2 cells in the presence of the whole scale of concentrations of carvacrol or thymol led in both cases to a significant protection of the cells studied toward DNA strand breaks induced by a potent oxidant hydrogen peroxide.
许多必需挥发油成分具有抗氧化活性，例如可作为合成抗氧化食品添加剂的天然替代品。然而，评估此类化合物的促氧化和毒性特性也很重要，因为它们的植物来源并不能确保其对包括人类在内的生物的安全性。因此，本研究的目的是在体外条件下，研究哺乳动物细胞与两种植物精油成分（香芹酚和百里香酚）长期（24小时）孵育后的细胞毒性、遗传毒性和DNA保护作用。在所有细胞系（人肝癌细胞HepG2、人结肠细胞Caco-2和仓鼠肺细胞V79）中，基于台盼蓝排斥法进行细胞毒性测试。仅在具有高集落形成能力的V79细胞中评估接种效率。通过标准碱性单细胞凝胶电泳法，测量用过氧化氢、香芹酚、百里香酚或香芹酚或百里香酚与过氧化氢的组合处理的细胞中诱导的DNA损伤量。台盼蓝排斥试验表明，香芹酚的细胞毒性略高于百里香酚，并且Caco-2细胞对香芹酚和百里香酚的耐受性略高于HepG2和V79细胞。在浓度=IC20-40时，所研究的化合物在人细胞HepG2或细胞Caco-2中均未诱导DNA链断裂。在香芹酚或百里香酚的全浓度范围内孵育HepG2和Caco-2细胞，在两种情况下均导致所研究的细胞对由强氧化剂过氧化氢诱导的DNA链断裂具有显著的保护作用。
