一种新型眼部给药装置的设计及其体外生物相容性

Design and in vitro biocompatibility of a novel ocular drug delivery device.

作者信息

Gooch Nathan, Burr Randon Michael, Holt Dolly J, Gale Bruce, Ambati Balamurali

机构信息

Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA.

Moran Eye Center, University of Utah, Salt Lake City, UT 84132, USA.

出版信息

J Funct Biomater. 2013 Jan 18;4(1):14-26. doi: 10.3390/jfb4010014.

DOI:10.3390/jfb4010014

PMID:24955828

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4030910/

Abstract

The capsule drug ring (CDR) is a reservoir and delivery agent, which is designed to be placed within the capsular bag during cataract surgery. Prototypes were manufactured by hot melt extrusion of Bionate II®, a polycarbonate urethane. The devices have been optimized using Avastin® as the drug of interest. In vitro biocompatibility was assessed with human lens epithelial cell (B-3), mouse macrophage (J774A.1) and mouse fibroblast (L-929) cell lines. Cell migration and proliferation were assessed after in vitro culture. Pro-inflammatory cytokines (i.e., MIP-1β, MIP-1α, MCP-1, IL-1β, TNF and TGF-β1) were quantified using cytometric bead array (CBA). Preliminary in vivo biocompatibility and pharmacokinetics testing has been performed in rabbits.

摘要

胶囊药物环（CDR）是一种储库和递送剂，设计用于在白内障手术期间放置在晶状体囊袋内。原型通过热熔融挤出聚碳酸酯聚氨酯Bionate II®制造。这些装置已使用阿瓦斯汀®作为目标药物进行了优化。使用人晶状体上皮细胞（B - 3）、小鼠巨噬细胞（J774A.1）和小鼠成纤维细胞（L - 929）细胞系评估体外生物相容性。体外培养后评估细胞迁移和增殖。使用细胞计数珠阵列（CBA）对促炎细胞因子（即MIP - 1β、MIP - 1α、MCP - 1、IL - 1β、TNF和TGF - β1）进行定量。已在兔子身上进行了初步的体内生物相容性和药代动力学测试。