Silge Anja, Schumacher Wilm, Rösch Petra, Da Costa Filho Paulo A, Gérard Cédric, Popp Jürgen
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany; InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, D-07743 Jena, Germany.
Analytical Sciences Department, Nestlé Research Center, Vers-chez-les-blanc, 1000 Lausanne 26, Switzerland.
Syst Appl Microbiol. 2014 Jul;37(5):360-7. doi: 10.1016/j.syapm.2014.05.007. Epub 2014 Jun 4.
The identification of Pseudomonas aeruginosa from samples of bottled natural mineral water by the analysis of subcultures is time consuming and other species of the authentic Pseudomonas group can be a problem. Therefore, this study aimed to investigate the influence of different aquatic environmental conditions (pH, mineral content) and growth phases on the cultivation-free differentiation between water-conditioned Pseudomonas spp. by applying Raman microspectroscopy. The final dataset was comprised of over 7500 single-cell Raman spectra, including the species Pseudomonas aeruginosa, P. fluorescens and P. putida, in order to prove the feasibility of the introduced approach. The collection of spectra was standardized by automated measurements of viable stained bacterial cells. The discrimination was influenced by the growth phase at the beginning of the water adaptation period and by the type of mineral water. Different combinations of the parameters were tested and they resulted in accuracies of up to 85% for the identification of P. aeruginosa from independent samples by applying chemometric analysis.
通过亚培养分析从瓶装天然矿泉水中鉴定铜绿假单胞菌耗时较长，而且真铜绿假单胞菌群的其他物种也可能成为问题。因此，本研究旨在通过应用拉曼光谱研究不同水生环境条件（pH值、矿物质含量）和生长阶段对适应水的假单胞菌属物种之间无培养分化的影响。最终数据集由超过7500个单细胞拉曼光谱组成，包括铜绿假单胞菌、荧光假单胞菌和恶臭假单胞菌等物种，以证明所引入方法的可行性。通过对活细菌细胞进行自动测量来标准化光谱收集。辨别受到水适应期开始时的生长阶段以及矿泉水类型的影响。测试了不同参数组合，通过化学计量分析从独立样本中鉴定铜绿假单胞菌的准确率高达85%。
