Yang Huiguang, Zhang Yunqing, Zhou Zhengming, Jiang Xuefeng, Shen Aidong
Department of Orthopaedics, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu 214400, P.R. China.
Oncol Lett. 2014 Jul;8(1):193-197. doi: 10.3892/ol.2014.2079. Epub 2014 Apr 22.
Osteosarcoma (OS) is a type of primary malignant bone tumor with a high propensity for local recurrence and distant metastasis. A previous study showed Snail-1 is highly expressed in OS cells. The present study aimed to investigate the association between the transcription factor Snai1 and E-cadherin in OS. SaOS OS cells were transfected either with a plasmid expressing short hairpin RNA (shRNA) specific for the Snai1-1 gene (SaOS-shRNA) or a negative control plasmid (SaOS-Mock). The expression levels of E-cadherin and Snai1-1 in the transfected and control cells were determined by quantitative polymerase chain reaction and western blot analysis. In addition, the study was extended to evaluate the migratory and invasive properties of the cells through a Transwell experiment. The results show that E-cadherin was expressed at a high level in the SaOS-shRNA cells, which were much less migratory and invasive than the control cells. Overexpression of Snai1-1 in OS is associated with tumor progression, possibly through the suppression of E-cadherin expression and induction of the process of epithelial-mesenchymal transition, which contributes to the proceeding invasion and metastasis of OS cells.
骨肉瘤（OS）是一种原发性恶性骨肿瘤，具有较高的局部复发和远处转移倾向。先前的一项研究表明，Snail-1在OS细胞中高表达。本研究旨在探讨转录因子Snai1与OS中E-钙黏蛋白之间的关联。将SaOS OS细胞用针对Snai1-1基因的短发夹RNA（shRNA）表达质粒（SaOS-shRNA）或阴性对照质粒（SaOS-Mock）进行转染。通过定量聚合酶链反应和蛋白质印迹分析确定转染细胞和对照细胞中E-钙黏蛋白和Snai1-1的表达水平。此外，该研究还通过Transwell实验扩展到评估细胞的迁移和侵袭特性。结果表明，E-钙黏蛋白在SaOS-shRNA细胞中高水平表达，其迁移和侵袭能力远低于对照细胞。OS中Snai1-1的过表达与肿瘤进展相关，可能是通过抑制E-钙黏蛋白表达并诱导上皮-间质转化过程，这有助于OS细胞的侵袭和转移。
