Prasad Ritika, Koch Biplob
Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Biomed Res Int. 2014;2014:753451. doi: 10.1155/2014/753451. Epub 2014 May 18.
Dendrobium, a genus of orchid, was found to possess useful therapeutic activities like anticancer, hypoglycaemic, antimicrobial, immunomodulatory, hepatoprotective, antioxidant, and neuroprotective activities. The study was aimed to evaluate the anticancer property of the ethanolic extract of Dendrobium formosum on Dalton's lymphoma. In vitro cytotoxicity was determined by MTT assay, apoptosis was determined by fluorescence microscopy, and cell cycle progression was analysed using flow cytometry; in vivo antitumor activity was performed in Dalton's lymphoma bearing mice. The IC50 value of ethanolic extract was obtained at 350 μg/mL in Dalton's lymphoma cells. Fluorescence microscopy analysis showed significant increase in apoptotic cell death in dose- and time-dependent manner which was further confirmed through the resulting DNA fragmentation. Further, flow cytometry analysis showed that the ethanolic extract arrests the cells in G2/M phase of the cell cycle. The in vivo anticancer activity study illustrates significant increase in the survival time of Dalton's lymphoma bearing mice on treatment with ethanolic extract when compared to control. These results substantiate the antitumor properties of ethanolic extract of Dendrobium formosum and suggest an alternative in treatment of cancer. Further studies are required regarding the isolation and characterization of bioactive components along with the analysis of molecular mechanism involved.
石斛是一种兰花属植物，被发现具有多种有益的治疗活性，如抗癌、降血糖、抗菌、免疫调节、保肝、抗氧化和神经保护活性。本研究旨在评估台湾石斛乙醇提取物对道尔顿淋巴瘤的抗癌特性。通过MTT法测定体外细胞毒性，通过荧光显微镜测定细胞凋亡，并使用流式细胞术分析细胞周期进程；在携带道尔顿淋巴瘤的小鼠中进行体内抗肿瘤活性实验。在道尔顿淋巴瘤细胞中，乙醇提取物的IC50值为350μg/mL。荧光显微镜分析显示，凋亡细胞死亡呈剂量和时间依赖性显著增加，DNA片段化进一步证实了这一点。此外，流式细胞术分析表明，乙醇提取物使细胞停滞在细胞周期的G2/M期。体内抗癌活性研究表明，与对照组相比，用乙醇提取物治疗的携带道尔顿淋巴瘤的小鼠存活时间显著延长。这些结果证实了台湾石斛乙醇提取物的抗肿瘤特性，并为癌症治疗提供了一种替代方法。关于生物活性成分的分离和表征以及所涉及的分子机制分析，还需要进一步研究。
