Immunology and Immunotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab 147 002, India.
Evid Based Complement Alternat Med. 2011;2011. doi: 10.1155/2011/765029. Epub 2010 Sep 26.
Ziziphus mauritiana (Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100-800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.
蜜枣（Ziziphus mauritiana）是一种具有民间药用价值的果树，可治疗多种疾病。本研究采用 MTT 法检测了蜜枣种子提取物对不同细胞系（HL-60、Molt-4、HeLa 和正常细胞系 HGF）的体外抗癌活性，并通过皮下接种艾氏腹水癌的瑞士白化病小鼠模型研究了该提取物的体内抗癌活性。结果表明，该提取物能明显抑制 HL-60 细胞的增殖。用不同浓度的提取物处理 HL-60 细胞后，Annexin 和 PI 结合实验显示提取物能以剂量依赖的方式诱导细胞凋亡。细胞周期分析显示，在浓度为 20μg/ml 及以上时，亚 G0 期细胞群显著增加。琼脂糖凝胶电泳显示，HL-60 细胞在与提取物孵育 3 h 后发生 DNA 片段化。该提取物在体内也表现出很强的抗癌活性。用不同剂量（100-800mg/kg 体重）的植物提取物处理皮下接种艾氏腹水癌的瑞士白化病小鼠，可显著降低肿瘤体积和活肿瘤细胞计数，提高血红蛋白含量、红细胞计数、平均存活时间、肿瘤抑制率和延长生命百分率。从脂质过氧化水平的降低和谷胱甘肽、过氧化氢酶和超氧化物歧化酶水平的升高可以看出，用提取物处理后的动物抗氧化状态增强。
