Central Department of Botany, Tribhuvan University, Kathmandu 44613, Nepal.
Annapurna Research Center, Kathmandu 44600, Nepal.
Biomolecules. 2019 Sep 12;9(9):478. doi: 10.3390/biom9090478.
is an epiphytic orchid found in south Asia including Nepal and China. This orchid species is widely used in traditional Chinese medicine (TCM) for the treatment of cancer, diabetes, cataracts, and fever. The objectives of the present research were to assess the antioxidant and cytotoxic properties of its stem's extracts with the identification of bioactive secondary metabolites. The antioxidant and cytotoxic activities were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, respectively, and compounds were identified using GC-MS (gas chromatography and mass spectrometry). Ethanol and acetone extracts scavenged 94.69 ± 0.10% and 93.41 ± 0.86% of DPPH free radicals, respectively. They showed 50% inhibition of DPPH free radicals (IC) at concentrations of 73.90 µg/mL and 99.44 µg/mL, which were found to be statistically similar to that of ascorbic acid (control). Chloroform extract inhibited the growth of 81.49 ± 0.43% of HeLa (human cervical carcinoma) cells and hexane extract inhibited the growth of 76.45 ± 4.26% of U251 (human glioblastoma) cells at 800 µg/mL concentration. These extracts showed 50% inhibition of cell growth (IC) toward both the HeLa and U251 cell lines at their high concentrations, which were found statistically significantly different from that of cisplatin drug (control). The above extracts showed antioxidant and cytotoxic properties, potentially due to the presence of tetracosane, triacontane, stigmasterol, and some phenol derivatives (2-methoxy-4-vinylphenol, 2-methoxy-5-(1-propenyl)-phenol, -mesyloxyphenol, and 2,6-dimethoxy-4-(2-propenyl)-phenol). This study explores the potential of this orchid in alternative medicine toward the development of drugs from its medicinally active compounds.
是一种附生兰花，分布于南亚，包括尼泊尔和中国。这种兰花在传统中药（TCM）中被广泛用于治疗癌症、糖尿病、白内障和发热。本研究的目的是评估其茎提取物的抗氧化和细胞毒性特性，并鉴定生物活性次生代谢物。抗氧化和细胞毒性活性分别采用 DPPH（2,2-二苯基-1-苦基肼）和 MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴盐）测定法进行评估，并用 GC-MS（气相色谱和质谱）鉴定化合物。乙醇和丙酮提取物分别清除了 94.69 ± 0.10%和 93.41 ± 0.86%的 DPPH 自由基。它们在 73.90 µg/mL 和 99.44 µg/mL 浓度下显示出对 DPPH 自由基的 50%抑制（IC），与抗坏血酸（对照）相当。氯仿提取物抑制 81.49 ± 0.43%的 HeLa（人宫颈癌细胞）生长，正己烷提取物抑制 76.45 ± 4.26%的 U251（人神经胶质瘤细胞）生长在 800 µg/mL 浓度下。这些提取物在高浓度下对 HeLa 和 U251 细胞系的细胞生长均显示出 50%的抑制（IC），与顺铂药物（对照）相比，这在统计学上有显著差异。上述提取物表现出抗氧化和细胞毒性特性，可能是由于存在二十四烷、三十烷、豆甾醇和一些酚类衍生物（2-甲氧基-4-乙烯基苯酚、2-甲氧基-5-(1-丙烯基)-苯酚、-甲氧基苯酚和 2,6-二甲氧基-4-(2-丙烯基)-苯酚）。本研究探索了这种兰花在替代医学中的潜力，以从其药用活性化合物中开发药物。
