Ding Yu, Ruan Dike, Luk Keith D K, He Qing, Wang Chaofeng
Department of Rehabilitation Medicine and Pain Management Center, Navy General Hospital, Beijing, China.
Department of Orthopaedics, Navy General Hospital, Beijing, China.
PLoS One. 2014 Jun 24;9(6):e100304. doi: 10.1371/journal.pone.0100304. eCollection 2014.
An animal experiment about intervertebral disc allograft.
To explore the feasibility to decellularize disc allografts treated by 6°Co Gamma Irradiation, and simultaneously, to assess the possibility to make use of the decellularized natural disc scaffold for disc degeneration biotherapy.
Studies of both animal and human disc allograft transplantation indicated that the disc allograft may serve as a scaffold to undertake the physiological responsibility of the segment.
Experiment in vitro: 48 discs of beagles were harvested and divided randomly into four groups including a control group and three irradiated groups. Immediate cell viability and biomechanical properties of the discs were checked and comparisons were made among these groups. Experiment in vivo: 24 beagles accepted single-level allografted disc treated with different doses of gamma irradiation. Plain X-rays and MRIs were taken before and after surgery. Then, the spinal columns were harvested en bloc from the sacrificed beagles and were examined morphologically.
There were significant differences of both the annulus fibrosus and nucleus pulposus immediate cell viabilities among the various groups. There were no obvious differences of the biomechanical properties among the four groups. The disc height and range of motion decreased significantly in all groups as time went on. The observed indexes in irradiated groups were much smaller than those in the control group, but the indexes in 18-kGy group were larger than those in 25-kGy and 50-kGy groups. Both MRI and macroscopic findings showed that the segmental degeneration in the control and 18-kGy group was less severe than that in 25-kGy and 50-kGy groups.
Gamma Irradiation can decellularize disc allograft successfully to provide natural scaffold for the study of degenerative disc disease therapy, and also can be used as an effective method to produce adjustable animal models.
一项关于椎间盘同种异体移植的动物实验。
探讨经6°Coγ射线辐照处理使椎间盘同种异体移植物去细胞化的可行性，同时评估利用去细胞化的天然椎间盘支架进行椎间盘退变生物治疗的可能性。
动物和人类椎间盘同种异体移植研究均表明，椎间盘同种异体移植物可作为承担节段生理功能的支架。
体外实验：收集48只比格犬的椎间盘，随机分为四组，包括一个对照组和三个辐照组。检测椎间盘的即刻细胞活力和生物力学性能，并在各组之间进行比较。体内实验：24只比格犬接受不同剂量γ射线辐照处理的单节段同种异体移植椎间盘。在手术前后拍摄X线平片和MRI。然后，从处死的比格犬身上整块取出脊柱并进行形态学检查。
各组之间纤维环和髓核的即刻细胞活力存在显著差异。四组之间的生物力学性能无明显差异。随着时间的推移，所有组的椎间盘高度和活动范围均显著降低。辐照组观察到的指标远小于对照组，但18 kGy组的指标大于25 kGy组和50 kGy组。MRI和大体观察结果均显示，对照组和18 kGy组的节段退变程度低于25 kGy组和50 kGy组。
γ射线辐照可成功使椎间盘同种异体移植物去细胞化，为椎间盘退变疾病治疗研究提供天然支架，也可作为建立可调节动物模型的有效方法。
