Furini C R G, Myskiw J C, Schmidt B E, Marcondes L A, Izquierdo I
Memory Center, Brain Institute, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690 - 2nd floor, 90610-000 Porto Alegre, RS, Brazil; National Institute of Translational Neuroscience (INNT), National Research Council of Brazil, Brazil.
Memory Center, Brain Institute, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690 - 2nd floor, 90610-000 Porto Alegre, RS, Brazil; National Institute of Translational Neuroscience (INNT), National Research Council of Brazil, Brazil.
Behav Brain Res. 2014 Sep 1;271:212-7. doi: 10.1016/j.bbr.2014.06.027. Epub 2014 Jun 21.
Memory consolidation is the process by which recently acquired information becomes stable and is modulated by different neurotransmitters depending on the structure involved and the nature of the memory. Here we evaluate the participation of both D1 and D5 dopamine receptors in the CA1 region of the hippocampus in the consolidation of the memory of two different tasks, object recognition (OR) and inhibitory avoidance (IA). For this, male rats with infusion cannulae stereotaxically implanted in the CA1 region of the dorsal hippocampus were trained in an OR task involving exposure to two different objects, or in a one-trial step-down IA task. At different times after the training, some of the animals received intrahippocampal infusions of the D1-family receptor antagonist SCH-23390. In a test session carried out 24h later, the animals that received infusions immediately or 60 min but not 180 min after the training showed impaired long-term memory. Since D1- and D5-subtypes engage different signaling pathways involving cAMP-dependent protein kinase (PKA) and protein kinase C (PKC), respectively, we assessed whether they participate distinctively in consolidation. The animals that received intra-CA1 infusions of the PKA inhibitor, Rp-cAMP, or the PKC inhibitor, Gö6976, immediately after OR or IA training had a long-term memory impairment and the amnesic effect caused by SCH-23390 was reversed when co-infused with activators of PKA (8Br-cAMP) or PKC (PMA). These results indicate that both D1 and D5 dopamine receptors are required in the CA1 region of the hippocampus for consolidation of the two tasks. This supports the notion of a commonality of consolidation mechanisms across tasks.
记忆巩固是一个过程，通过这个过程，最近获取的信息变得稳定，并根据所涉及的结构和记忆的性质由不同的神经递质进行调节。在这里，我们评估了D1和D5多巴胺受体在海马体CA1区域对两种不同任务（物体识别（OR）和抑制性回避（IA））记忆巩固中的参与情况。为此，将带有灌注套管并通过立体定位植入背侧海马体CA1区域的雄性大鼠训练进行涉及接触两个不同物体的OR任务，或进行一次性逐步下降的IA任务。在训练后的不同时间，一些动物接受海马体内灌注D1家族受体拮抗剂SCH-23390。在24小时后进行的测试环节中，在训练后立即或60分钟而非180分钟接受灌注的动物表现出长期记忆受损。由于D1和D5亚型分别参与涉及环磷酸腺苷依赖性蛋白激酶（PKA）和蛋白激酶C（PKC）的不同信号通路，我们评估了它们是否在巩固过程中发挥不同作用。在OR或IA训练后立即接受CA1内灌注PKA抑制剂Rp-cAMP或PKC抑制剂Gö6976的动物出现长期记忆受损，并且当与PKA激活剂（8Br-cAMP）或PKC激活剂（PMA）共同灌注时，SCH-23390引起的遗忘效应被逆转。这些结果表明，海马体CA1区域的D1和D5多巴胺受体对于这两项任务的巩固都是必需的。这支持了不同任务巩固机制具有共性的观点。
