de Landeta Ana Belén, Medina Jorge H, Katche Cynthia
CONICET-Universidad de Buenos Aires, Instituto de Biología Celular y Neurociencia "Prof. E. De Robertis" (IBCN), Buenos Aires, Argentina.
Universidad de Buenos Aires, Facultad de Medicina, Buenos Aires, Argentina.
Front Behav Neurosci. 2022 Jul 7;16:922971. doi: 10.3389/fnbeh.2022.922971. eCollection 2022.
The retrosplenial cortex (RSC) has been widely related to spatial and contextual memory. However, we recently demonstrated that the anterior part of the RSC (aRSC) is required for object recognition (OR) memory consolidation. In this study, we aimed to analyze the requirement of dopaminergic inputs into the aRSC for OR memory consolidation in male rats. We observed amnesia at 24-h long-term memory when we infused SCH23390, a D1/D5 dopamine receptors antagonist, into aRSC immediately after OR training session. However, the same infusion had no effect on OR short-term memory. Then, we analyzed whether the ventral tegmental area (VTA) is necessary for OR consolidation. VTA inactivation by intra-VTA administration of muscimol, a GABA agonist, immediately after an OR training session induced amnesia when animals were tested at 24 h. Moreover, we observed that this VTA inactivation-induced amnesia was reversed by the simultaneous intra-aRSC delivery of SKF38393, a D1/D5 receptor agonist. Altogether, our results suggest that VTA dopaminergic inputs to aRSC play an important modulatory role in OR memory consolidation.
压后皮质（RSC）与空间和情境记忆广泛相关。然而，我们最近证明，RSC的前部（aRSC）对于物体识别（OR）记忆巩固是必需的。在本研究中，我们旨在分析雄性大鼠aRSC中多巴胺能输入对OR记忆巩固的需求。在OR训练后立即向aRSC注入D1/D5多巴胺受体拮抗剂SCH23390时，我们观察到24小时长期记忆出现失忆。然而，相同的注入对OR短期记忆没有影响。然后，我们分析了腹侧被盖区（VTA）对OR巩固是否必要。在OR训练后立即通过向VTA内注射GABA激动剂蝇蕈醇使VTA失活，当在24小时对动物进行测试时诱导失忆。此外，我们观察到这种VTA失活诱导的失忆通过同时向aRSC内递送D1/D5受体激动剂SKF38393而得到逆转。总之，我们的结果表明，VTA向aRSC的多巴胺能输入在OR记忆巩固中起重要的调节作用。
