Liu Ying, Han Xiao-Jie, Liu Ming-Hui, Wang Shu-Yu, Jia Chan-Wei, Yu Lan, Ren Guoqing, Wang Li, Li Wei
Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University , Beijing, China .
Cell Reprogram. 2014 Aug;16(4):276-80. doi: 10.1089/cell.2013.0081. Epub 2014 Jun 24.
Our objective was to observe the effectiveness of the calcium ionophore A23187 or strontium chloride on the activation and subsequent embryonic development of 3-day-old human unfertilized oocytes after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). A total of 279 3-day-old unfertilized oocytes after IVF or ICSI were randomized to be activated by the calcium ionophore A23187 (n=138) or strontium chloride (n=141). The activated oocytes were cultured in vitro for 3-5 days. Activation rate, pronucleus formation, cleavage rate, and developmental potential of parthenotes during culture were evaluated. A total of 170 unfertilized oocytes were activated; 65 developed to cleavage stage, 19 developed to greater than the eight-cell stage, and five blastocysts were obtained. The activation rate of the calcium ionophore A23187 group was higher than that of the strontium chloride group (75.4% and 46.8%, respectively; p<0.05); there was significant difference between two groups (p<0.05). Among the 44 cleaved oocytes in the calcium ionophore A23187 group, eight developed to the two- to four-cell stage, 17 developed to the five- to eight-cell stage, 15 developed to greater than the eight-cell stage, and four blastocysts were obtained. Among the 21 cleaved oocytes in the strontium chloride group, six developed to the two- to four- cell stage, 10 developed to the five- to eight-cell stage, four developed to greater than the eight-cell stage, and one blastocyst was obtained. Three-day-old unfertilized human oocytes after IVF or ICSI could be activated by the calcium ionophore A23187 or strontium chloride, and a small part of parthenogenetic embryos developed into blastocysts. The treatment with the calcium ionophore A23187 was better than that of strontium chloride in respect to the activation rate of 3-day-old unfertilized human oocytes after IVF or ICSI.
我们的目的是观察钙离子载体A23187或氯化锶对体外受精（IVF）或卵胞浆内单精子注射（ICSI）后3日龄未受精人卵母细胞的激活及随后胚胎发育的有效性。总共279枚IVF或ICSI后的3日龄未受精人卵母细胞被随机分为两组，分别用钙离子载体A23187（n = 138）或氯化锶（n = 141）激活。激活后的卵母细胞在体外培养3 - 5天。评估孤雌胚胎在培养过程中的激活率、原核形成、卵裂率和发育潜能。总共170枚未受精的卵母细胞被激活；65枚发育到卵裂期，19枚发育到八细胞期以上，获得了5个囊胚。钙离子载体A23187组的激活率高于氯化锶组（分别为75.4%和46.8%；p<0.05）；两组间存在显著差异（p<0.05）。在钙离子载体A23187组的44枚卵裂卵母细胞中，8枚发育到二至四细胞期，17枚发育到五至八细胞期，15枚发育到八细胞期以上，获得了4个囊胚。在氯化锶组的21枚卵裂卵母细胞中，6枚发育到二至四细胞期，10枚发育到五至八细胞期，4枚发育到八细胞期以上，获得了1个囊胚。IVF或ICSI后的3日龄未受精人卵母细胞可被钙离子载体A23187或氯化锶激活，且一小部分孤雌胚胎发育成了囊胚。就IVF或ICSI后3日龄未受精人卵母细胞的激活率而言，钙离子载体A23187的处理效果优于氯化锶。
