在发生自发性肝炎的LEC大鼠肝脏中，异常高倍体细胞核的积累。
Accumulation of abnormally high ploid nuclei in the liver of LEC rats developing spontaneous hepatitis.
作者信息
Fujimoto Y, Oyamada M, Hattori A, Takahashi H, Sawaki M, Dempo K, Mori M, Nagao M
机构信息
Department of Pathology, Sapporo Medical College.
出版信息
Jpn J Cancer Res. 1989 Jan;80(1):45-50. doi: 10.1111/j.1349-7006.1989.tb02243.x.
Enlarged hepatocytes with huge nuclei were found in LEC rats with hereditary hepatitis. Flow cytometric analysis of the DNA content of nuclei from jaundiced LEC rats revealed the presence of very high polyploids, such as 32n and 64n. At the age of 12 weeks, before the onset of hepatitis, 8n polyploid nuclei were more frequent in LEC rats than in LEA rats, a sibling line of LEC rats. Binucleated hepatocytes were also more frequent in LEC rats than in LEA rats at week 4. Bi-, tri- and tetra-nucleated cells whose nuclei were sometimes different in size were observed when jaundice became manifest. The number of proliferating liver cells, determined by pulse labeling with 5-bromo-2'-deoxyuridine (BrdU), was higher in LEC rats than in LEA rats at 2, 4, 8, 12 and 14 weeks, with a maximum at week 4. A remarkable increase of BrdU uptake was observed at week 16, when jaundice developed. The possible involvement of abnormal cytokinesis and kariokinesis in the manifestation of hepatitis was suggested.
在患有遗传性肝炎的LEC大鼠中发现了具有巨大细胞核的肿大肝细胞。对黄疸型LEC大鼠细胞核的DNA含量进行流式细胞术分析，结果显示存在非常高的多倍体，如32n和64n。在12周龄时，即肝炎发作前，LEC大鼠中8n多倍体细胞核的出现频率高于其同系的LEA大鼠。在第4周时，双核肝细胞在LEC大鼠中的出现频率也高于LEA大鼠。当黄疸出现时，观察到双核、三核和四核细胞，其细胞核大小有时不同。通过用5-溴-2'-脱氧尿苷（BrdU）进行脉冲标记测定的增殖肝细胞数量，在第2、4、8、12和14周时LEC大鼠高于LEA大鼠，在第4周时达到最大值。在第16周黄疸出现时，观察到BrdU摄取显著增加。提示异常胞质分裂和核分裂可能与肝炎的表现有关。
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