Agnihotri Naveen, Pal Lokesh, Thakur Manish, Kumar Pravin
Blood Bank, Department of Transfusion Medicine, Fortis Hospital, Shalimar Bagh, Delhi, India.
Blood Transfus. 2014 Oct;12(4):520-6. doi: 10.2450/2014.0231-13. Epub 2014 Jun 5.
Red blood cell (RBC) transfusions are given as "number of units" without considering the haemoglobin (Hb) content of these units. Donor factors influencing Hb level in whole blood donors and, ultimately, in RBC units have not been studied.
Donor data for a period of 1.5 years were retrospectively analysed and the effects of age, gender and weight on the Hb level of the donors were determined. The correlation between donor's Hb concentration with total Hb in the RBC unit was analysed. Additionally, actual Hb content of 125 RBC units was determined. The total Hb content of these RBC units was also mathematically calculated based on the blood donors' Hb. The ability of this mathematically calculated Hb to predict actual Hb content per RBC unit was then analysed.
The mean Hb level in female donors was 1.79 g/dL lower than in the male donors (p<0.001). Increasing age was associated with a lower mean Hb in the donors (p<0.01), while a higher body weight correlated weakly (r=0.06) but significantly with increased mean Hb (p<0.01). Logistic regression analysis showed that in blood donors, female gender had a stronger influence on lowering the mean Hb than either older age or lower weight. A variation of nearly 100% (42.3-80.8 g Hb per unit) was seen in the total Hb content of the RBC units tested. Mathematically calculated Hb content correlated well (r=0.6; p<0.01) with the actual Hb content of the RBC units.
We demonstrated the effect of gender, age and weight on Hb levels in whole blood donors. Dissimilarities in the donor Hb caused nearly 100% variations in the Hb content of the RBC units. It would, therefore, be prudent to label RBC units with their total Hb content. This total Hb content can be predicted fairly accurately from the donor's pre-donation Hb level.
红细胞（RBC）输血是以“单位数量”进行的，而未考虑这些单位中的血红蛋白（Hb）含量。影响全血捐献者血红蛋白水平以及最终影响红细胞单位中血红蛋白水平的捐献者因素尚未得到研究。
对1.5年期间的捐献者数据进行回顾性分析，确定年龄、性别和体重对捐献者血红蛋白水平的影响。分析捐献者血红蛋白浓度与红细胞单位中总血红蛋白之间的相关性。此外，测定了125个红细胞单位的实际血红蛋白含量。还根据献血者的血红蛋白对这些红细胞单位的总血红蛋白含量进行了数学计算。然后分析这种通过数学计算得出的血红蛋白预测每个红细胞单位实际血红蛋白含量的能力。
女性捐献者的平均血红蛋白水平比男性捐献者低1.79 g/dL（p<0.001）。年龄增加与捐献者平均血红蛋白水平降低相关（p<0.01），而体重增加与平均血红蛋白水平升高的相关性较弱（r=0.06）但显著相关（p<0.01）。逻辑回归分析表明，在献血者中，女性性别对降低平均血红蛋白的影响比年龄较大或体重较轻更为显著。在所测试的红细胞单位中，总血红蛋白含量的变化近100%（每单位42.3 - 80.8 g血红蛋白）。通过数学计算得出的血红蛋白含量与红细胞单位的实际血红蛋白含量相关性良好（r=0.6；p<0.01）。
我们证明了性别、年龄和体重对全血捐献者血红蛋白水平的影响。捐献者血红蛋白的差异导致红细胞单位中血红蛋白含量出现近100%的变化。因此，明智的做法是在红细胞单位上标注其总血红蛋白含量。这种总血红蛋白含量可以根据捐献者献血前的血红蛋白水平相当准确地预测出来。
