Department of Bioresource Sciences, Andong National University, Andong 760749, South Korea.
BMC Complement Altern Med. 2014 Jun 25;14:200. doi: 10.1186/1472-6882-14-200.
Root bark of mulberry (Morus alba L.) has been used in herbal medicine as anti-phlogistic, liver protective, kidney protective, hypotensive, diuretic, anti-cough and analgesic agent. However, the anti-cancer activity and the potential anti-cancer mechanisms of mulberry root bark have not been elucidated. We performed in vitro study to investigate whether mulberry root bark extract (MRBE) shows anti-inflammatory and anti-cancer activity.
In anti-inflammatory activity, NO was measured using the griess method. iNOS and proteins regulating NF-κB and ERK1/2 signaling were analyzed by Western blot. In anti-cancer activity, cell growth was measured by MTT assay. Cleaved PARP, ATF3 and cyclin D1 were analyzed by Western blot.
In anti-inflammatory effect, MRBE blocked NO production via suppressing iNOS over-expression in LPS-stimulated RAW264.7 cells. In addition, MRBE inhibited NF-κB activation through p65 nuclear translocation via blocking IκB-α degradation and ERK1/2 activation via its hyper-phosphorylation. In anti-cancer activity, MRBE deos-dependently induced cell growth arrest and apoptosis in human colorectal cancer cells, SW480. MRBE treatment to SW480 cells activated ATF3 expression and down-regulated cyclin D1 level. We also observed that MRBE-induced ATF3 expression was dependent on ROS and GSK3β. Moreover, MRBE-induced cyclin D1 down-regulation was mediated from cyclin D1 proteasomal degradation, which was dependent on ROS.
These findings suggest that mulberry root bark exerts anti-inflammatory and anti-cancer activity.
桑树根皮（Morus alba L.）在草药学中被用作抗炎、护肝、护肾、降血压、利尿、止咳和镇痛剂。然而，桑树根皮的抗癌活性及其潜在的抗癌机制尚未阐明。我们进行了体外研究，以探讨桑树根皮提取物（MRBE）是否具有抗炎和抗癌活性。
在抗炎活性方面，使用格里斯法测量 NO。通过 Western blot 分析 iNOS 和调节 NF-κB 和 ERK1/2 信号通路的蛋白质。在抗癌活性方面，通过 MTT 测定法测量细胞生长。通过 Western blot 分析裂解的 PARP、ATF3 和细胞周期蛋白 D1。
在抗炎作用方面，MRBE 通过抑制 LPS 刺激的 RAW264.7 细胞中 iNOS 的过表达来阻断 NO 的产生。此外，MRBE 通过抑制 IκB-α降解来抑制 NF-κB 激活，通过其过度磷酸化来抑制 ERK1/2 激活。在抗癌活性方面，MRBE 依赖性诱导人结直肠癌细胞 SW480 的细胞生长停滞和凋亡。MRBE 处理 SW480 细胞激活 ATF3 表达并下调细胞周期蛋白 D1 水平。我们还观察到，MRBE 诱导的 ATF3 表达依赖于 ROS 和 GSK3β。此外，MRBE 诱导的细胞周期蛋白 D1 下调是通过细胞周期蛋白 D1 蛋白酶体降解介导的，这依赖于 ROS。
这些发现表明桑树根皮具有抗炎和抗癌活性。
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