用于白蛋白固定的改性磁铁矿纳米颗粒的表征

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

Bordbar A K, Rastegari A A, Amiri R, Ranjbakhsh E, Abbasi M, Khosropour A R

Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran ; Department of Biotechnology, Faculty of New Science and Technology, University of Isfahan, Isfahan 81746-73441, Iran.

Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.

Biotechnol Res Int. 2014;2014:705068. doi: 10.1155/2014/705068. Epub 2014 May 22.

Magnetite Fe3O4 nanoparticles (NPs) were prepared by chemical coprecipitation method. Silica-coated magnetite NPs were prepared by sol-gel reaction, subsequently coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction, and then were activated with 2,4,6-trichloro-1,3,5-triazine (TCT) and covalently immobilized with bovine serum albumin (BSA). The size and structure of the particles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and dynamic light scattering (DLS) techniques. The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR). XRD analysis showed that the binding process has not done any phase change to Fe3O4. The immobilization time for this process was 4 h and the amount of immobilized BSA for the initial value of 1.05 mg BSA was about 120 mg/gr nanoparticles. Also, the influences of three different buffer solutions and ionic strength on covalent immobilization were evaluated.

采用化学共沉淀法制备了磁铁矿Fe3O4纳米颗粒（NPs）。通过溶胶 - 凝胶反应制备了二氧化硅包覆的磁铁矿纳米颗粒，随后通过硅烷化反应包覆3 - 氨丙基三乙氧基硅烷（APTES），然后用2,4,6 - 三氯 - 1,3,5 - 三嗪（TCT）活化并与牛血清白蛋白（BSA）共价固定。通过透射电子显微镜（TEM）、X射线粉末衍射（XRD）和动态光散射（DLS）技术对颗粒的尺寸和结构进行了表征。通过傅里叶变换红外光谱（FT - IR）确认了固定化。XRD分析表明，结合过程未对Fe3O4产生任何相变。该过程的固定时间为4小时，对于初始值为1.05 mg BSA，固定化的BSA量约为120 mg/克纳米颗粒。此外，还评估了三种不同缓冲溶液和离子强度对共价固定化的影响。