Can Keziban, Ozmen Mustafa, Ersoz Mustafa
Department of Chemistry, Selcuk University, Konya 42075, Turkey.
Colloids Surf B Biointerfaces. 2009 Jun 1;71(1):154-9. doi: 10.1016/j.colsurfb.2009.01.021. Epub 2009 Feb 11.
Magnetite nanoparticles (NPs) were synthesized by co-precipitating Fe(2+) and Fe(3+) in an ammonia solution. Magnetite NPs coated with 3-aminopropyltriethoxysilane (APTES) were prepared by silanization reaction. After activated with glutaraldehyde (GA), human serum albumin was covalently immobilized on these magnetic NPs. The influence of three different buffer solutions on covalent immobilization was studied. phosphate buffer saline solution (PBS) was shown the highest binding ability for immobilization process compared to other buffer solutions. The morphology and properties of these magnetic NPs were examined by transmission electron eicroscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA) and magnetization measurement.
通过在氨溶液中共沉淀Fe(2+)和Fe(3+)合成了磁铁矿纳米颗粒（NPs）。通过硅烷化反应制备了涂覆有3-氨丙基三乙氧基硅烷（APTES）的磁铁矿纳米颗粒。用戊二醛（GA）活化后，将人血清白蛋白共价固定在这些磁性纳米颗粒上。研究了三种不同缓冲溶液对共价固定的影响。与其他缓冲溶液相比，磷酸盐缓冲盐水溶液（PBS）在固定过程中表现出最高的结合能力。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、热重分析（TGA）和磁化测量对这些磁性纳米颗粒的形态和性质进行了研究。
