Mala Wanida, Chomvarin Chariya, Alam Munirul, Rashed Shah M, Faksri Kiatichai, Angkititrakul Sunpetch
Southeast Asian J Trop Med Public Health. 2014 Jan;45(1):103-12.
Vibrio vulnificus can cause septicemia, wound infection and gastroenteritis. The most severe infections are related to consumption of raw or undercooked seafood. Virulence genes, biomarkers, antimicrobial resistance, and genetic relationships among V vulnificus isolated from clinical and environmental sources in Thailand have not hitherto been investigated. ViuB encoding vulnibactin siderophore was detected in 33% and 50% of clinical and environmental (cockle) V. vulnificus isolates, respectively, and capsular polysaccharide allele 1 in 67% and 75% of clinical and environmental isolates, respectively. Analysis of the 16 S rDNA gene revealed that type B was the most frequent in both clinical and environmental isolates (67%) whereas the non type-able (30%) was detected only in environmental isolates. The virulence-correlated gene (vcg) with both type C and E together was the most frequently found among the clinical (67%) and environmental (72%) isolates. Pulsed-field gel electrophoresis differentiated V vulnificus into 2 clusters; most cockle samples (83%) and all clinical isolates grouped into cluster II, indicating a possible clonal relationship between V. vulnificus isolated from patients and cockles. Only 20% of environmental isolates were resistant to ampicillin. These studies suggest that V vulnificus isolated from cockles has virulence genes similar to those in clinical isolates and thus may have the potential of causing disease.
创伤弧菌可引起败血症、伤口感染和肠胃炎。最严重的感染与食用生的或未煮熟的海鲜有关。泰国临床和环境来源分离出的创伤弧菌的毒力基因、生物标志物、抗菌耐药性以及遗传关系迄今尚未得到研究。分别在33%的临床创伤弧菌分离株和50%的环境（鸟蛤）创伤弧菌分离株中检测到编码铁载体弧菌素的ViuB，在67%的临床分离株和75%的环境分离株中检测到荚膜多糖等位基因1。对16S rDNA基因的分析表明，B型在临床和环境分离株中最为常见（67%），而非分型（30%）仅在环境分离株中检测到。C型和E型毒力相关基因（vcg）在临床（67%）和环境（72%）分离株中最为常见。脉冲场凝胶电泳将创伤弧菌分为2个簇；大多数鸟蛤样本（83%）和所有临床分离株归为簇II，表明从患者和鸟蛤中分离出的创伤弧菌之间可能存在克隆关系。仅20%的环境分离株对氨苄青霉素耐药。这些研究表明，从鸟蛤中分离出的创伤弧菌具有与临床分离株相似的毒力基因，因此可能具有致病潜力。
