Serratore Patrizia, Zavatta Emanuele, Fiocchi Eleonora, Serafini Emanuele, Serraino Andrea, Giacometti Federica, Bignami Giorgia
Department of Veterinary Medical Science, Alma Mater Studiorum- University of Bologna, Bologna, Italy.
Ital J Food Saf. 2017 Oct 20;6(4):6843. doi: 10.4081/ijfs.2017.6843.
is a Gram-negative bacterium, commonly found in estuarine and coastal habitats, that can infect humans through seafood consumption or wound exposure. This study represents the first attempt to correlate the genotype of strains isolated in the north-western Adriatic Sea coastal area, with their antimicrobial susceptibility patterns. On the whole, 40 strains, isolated from shellfish (n=20), different coastal water bodies (n=19), and the blood of a turtle (n=1), were utilized. All strains were positive for the species-specific genes A and , with high variability for other markers: 55% (22 out of 40) resulted of the environmental (E) genotype (E, 16S rRNA type A, CPS2 or CPS0), 10% (4 out of 40) of the clinical (C) genotype (C, 16S rRNA type B, CPS1), and 35% (14 out of 40) of the mixed (M) genotype, possessing both E and C markers. The antimicrobial susceptibility was assayed by the diffusion method on agar, according to the Clinical Laboratory Standards Institute (CLSI), utilizing the following commercial disks (Oxoid): ampicillin (AMP), ampicillin- sulbactam (SAM), piperacillin (PRL), cefazolin (KZ), cefotaxime(CTX), ceftazidime (CAZ), imipenem (IPM), meropenem (MEM), amikacin (AK), gentamicin(CN), tetracycline(TE), ciprofloxacin (CIP), levofloxacin (LEV), trimethoprim-sulfamethoxazole (SXT), and chloramphenicol (C). 75% of the strains, (n=30) including all C strains, was sensitive to all the tested antibiotics, whereas E strains showed intermediate sensitivity to AK (2 strains), CIP and CAZ (1 strain), TE (1 strain) and resistance to KZ (1 strain), and 4 M strains showed I to AK.
是一种革兰氏阴性菌，常见于河口和沿海栖息地，可通过食用海鲜或伤口暴露感染人类。本研究首次尝试将亚得里亚海西北部沿海地区分离的菌株基因型与其抗菌药敏模式相关联。总体而言，共使用了40株菌株，分别从贝类（n = 20）、不同的沿海水体（n = 19）以及一只海龟的血液（n = 1）中分离得到。所有菌株的物种特异性基因A和 均为阳性，其他标记具有高度变异性：55%（40株中的22株）为环境（E）基因型（E，16S rRNA A型，CPS2或CPS0），10%（40株中的4株）为临床（C）基因型（C，16S rRNA B型，CPS1），35%（40株中的14株）为混合（M）基因型，同时具有E和C标记。根据临床实验室标准协会（CLSI）的方法，采用琼脂扩散法对抗菌药敏性进行检测，使用以下商业药敏纸片（Oxoid）：氨苄西林（AMP）、氨苄西林-舒巴坦（SAM）、哌拉西林（PRL）、头孢唑林（KZ）、头孢噻肟（CTX）、头孢他啶（CAZ）、亚胺培南（IPM）、美罗培南（MEM）、阿米卡星（AK）、庆大霉素（CN）、四环素（TE）、环丙沙星（CIP）、左氧氟沙星（LEV）、甲氧苄啶-磺胺甲恶唑（SXT）和氯霉素（C）。75%的菌株（n = 30），包括所有C基因型菌株，对所有测试抗生素敏感，而E基因型菌株对AK（2株）、CIP和CAZ（1株）、TE（1株）表现出中度敏感性，对KZ（1株）耐药，4株M基因型菌株对AK表现出I类反应。
