Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Lett Appl Microbiol. 2020 Jul;71(1):61-69. doi: 10.1111/lam.13232. Epub 2019 Nov 3.
The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were detected using gyrB sequencing. The phenotypic pathogenicity revealed that the DNase, amylase and phospholipase activities were 100%, while lipase, slime production, gelatinase and caseinase were detected in 72, 88, 88 and 81% of the isolates respectively. The PCR amplification for the detection of V. parahaemolyticus species-specific tdh, tlh, trh and toxR genes were positive in 4 (13%), 16 (50%), 0 (0%) and 4 (13%) isolates respectively. The V. alginolytuicus species-specific tdh, tlh, trh, toxR and vac genes were carried by 15 (47%), 29 (91%), 0 (0%), 15 (47%) and 25 (78%) of the isolates respectively. In addition, multidrug resistance was observed by 27 (84%) isolates, whereas higher resistant rates were observed against ampicillin, piperacillin, streptomycin and cephalothin. The occurrence of bla (78%), bla (40%), bla (22%) and aac(6')-Ib (94%) were prevalent, while strAB, tetB, aphAI-IAB, intl1 and aadA1 gene cassettes were also detected. The results signify the potential health risks resulting from the consumption of raw cockles in Korea. SIGNIFICANCE AND IMPACT OF THE STUDY: Vibrios are well known to cause human infections following consumption of raw or undercooked seafood. This phenomenon has undoubtedly increased the number of health issues over the past few years in Korea. Among the identified Vibrio spp., we could detect V. diabolicus and V. harveyi for the first time in marketed cockles in Korea. The presence of species-specific genes (tdh-VA, tlh-VP, tlh-VA and toxR-VA) in V. diabolicus exhibits the close genetic affinity among V. parahaemolyticus and V. alginolyticus. Furthermore, the prevalence of extended-spectrum β-lactamases (ESBLs) and other antibiotic resistance genes along with multidrug resistance signifies the potential threat for consumers.
本研究旨在调查从韩国市场销售的文蛤（Tegillarca granosa）中分离的弧菌属（Vibrio spp.）的发病率、毒力和抗生素特性。通过 gyrB 测序共检测到 32 株弧菌属，包括副溶血弧菌（n = 4）、溶藻弧菌（n = 11）、狄氏弧菌（n = 14）和哈维氏弧菌（n = 3）。表型致病性表明，DNase、淀粉酶和磷脂酶活性为 100%，而脂肪酶、黏液生成、明胶酶和酪蛋白酶活性分别在 72%、88%、88%和 81%的分离株中检测到。PCR 扩增检测副溶血弧菌种特异性 tdh、tlh、trh 和 toxR 基因，4 株（13%）、16 株（50%）、0 株（0%）和 4 株（13%）的分离株呈阳性。溶藻弧菌种特异性 tdh、tlh、trh、toxR 和 vac 基因分别由 15 株（47%）、29 株（91%）、0 株（0%）、15 株（47%）和 25 株（78%）的分离株携带。此外，27 株（84%）分离株表现出多药耐药性，而对氨苄西林、哌拉西林、链霉素和头孢噻吩的耐药率较高。bla（78%）、bla（40%）、bla（22%）和 aac(6')-Ib（94%）的发生率较高，同时还检测到 strAB、tetB、aphAI-IAB、intl1 和 aadA1 基因盒。这些结果表明，在韩国食用生文蛤可能会带来健康风险。研究的意义和影响：弧菌属众所周知，食用生的或未煮熟的海鲜会导致人类感染。在过去的几年里，这种现象无疑在韩国增加了许多健康问题。在所鉴定的弧菌属中，我们首次在韩国市场销售的文蛤中检测到狄氏弧菌和哈维氏弧菌。狄氏弧菌中存在种特异性基因（tdh-VA、tlh-VP、tlh-VA 和 toxR-VA），表明副溶血弧菌和溶藻弧菌之间具有密切的遗传亲和力。此外，ESBLs 和其他抗生素耐药基因的流行以及多药耐药性表明对消费者构成潜在威胁。
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