Huang C Q, Liu G D, Bai C J, Wang W Q, Tang J
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou, 571737, Hainan, China,
Mol Biol Rep. 2014 Sep;41(9):5923-9. doi: 10.1007/s11033-014-3467-0. Epub 2014 Jun 26.
Sequence-related amplified polymorphism (SRAP) is a new molecular marker technology developed based on polymerase chain reaction. The authenticity of 84 progenies of 8 hybrid combinations of Stylosanthes guianensis was identified by SRAP markers to select the true hybrids used in the present study. A total of 35 SRAP primer combinations were selected from the parents of 8 hybrid combinations. The selected polymorphism primer combinations were applied to identify the authenticity of all progenies. The male parents of the primer combinations had specific markers, whereas the female parents did not. 68 progenies exhibited male parent-specific bands, which were identified as true hybrids. The rest of the progenies were considered self-hybrids because of the absence of male parent-specific bands. The results of hybrid identification provided solid evidence for further studies of hybrids and demonstrated SRAP molecular markers as a useful technology for assessing the purity of S. guianensis hybrids.
序列相关扩增多态性（SRAP）是一种基于聚合酶链反应开发的新型分子标记技术。利用SRAP标记对8个圭亚那柱花草杂交组合的84个后代进行真伪鉴定，以筛选出本研究中使用的真杂种。从8个杂交组合的亲本中总共筛选出35对SRAP引物组合。将筛选出的多态性引物组合用于鉴定所有后代的真伪。引物组合的父本具有特异性标记，而母本没有。68个后代出现父本特异性条带，被鉴定为真杂种。其余后代由于没有父本特异性条带而被认为是自交杂种。杂种鉴定结果为进一步研究杂种提供了确凿证据，并证明SRAP分子标记是评估圭亚那柱花草杂种纯度的一种有用技术。
