Xie Xin-Ming, Zhou Feng, Zhang Xiang-Qian, Zhang Ju-Ming
College of Agriculture, South China Agricultural University, Guangzhou 510642, People's Republic of China.
J Genet. 2009 Dec;88(3):281-90. doi: 10.1007/s12041-009-0041-y.
Genetic variability and relationships among elephant grass cultivars were estimated by the SRAP (sequence-related amplified polymorphism) assay. A total of 60 individuals collected from five cultivars in China were analysed. Sixty-two selected primer combinations generated 1395 bands, with an average of 22.5 per primer combination. The average value of percentage of polymorphic bands (PPB) was 72.8% at species level. The PPB was from 15.2% to 75%, with an average of 39.6% at cultivar level. H(POP), within-cultivar Shannon's index was 1.738 at cultivar level; at species level, the Shannon's index (H(SP)) was 3.880. An assessment of diversity between cultivars (H(SP)-H(POP))/H(SP)] indicated that most of the diversity (55.2%) was detected among cultivars, and only 44.8% was within cultivars in total genetic variation. According to UPGMA dendrogram, the five cultivars were clustered into three main groups. One group included MT-1 and Mott with a bootstrap support of 100%, another consisted of Huanan and N51 with a bootstrap support of 81%, and last one was only Guimu-1. The results indicate that the MT-1 and Mott have a closest genetic relationship; Huanan and N51 possess a relatively close relationship, and Guimu-1 is the most distinct from the other four cultivars.
通过序列相关扩增多态性（SRAP）分析评估了象草品种间的遗传变异性和关系。对从中国五个品种收集的总共60个个体进行了分析。62个选定的引物组合产生了1395条带，每个引物组合平均产生22.5条带。在物种水平上，多态性条带百分比（PPB）的平均值为72.8%。在品种水平上，PPB为15.2%至75%，平均值为39.6%。在品种水平上，品种内香农指数H（POP）为1.738；在物种水平上，香农指数H（SP）为3.880。对品种间多样性的评估[（H（SP）-H（POP））/H（SP）]表明，在总遗传变异中，大部分多样性（55.2%）存在于品种间，只有44.8%存在于品种内。根据UPGMA聚类图，五个品种被聚类为三个主要类群。一个类群包括MT-1和Mott，自展支持率为100%，另一个由华南和N51组成，自展支持率为81%，最后一个只有桂牧1号。结果表明，MT-1和Mott的遗传关系最密切；华南和N51具有相对密切的关系，桂牧1号与其他四个品种差异最大。
