Nishise Shoichi, Abe Yasuhiko, Nomura Eiki, Sato Takeshi, Sasaki Yu, Iwano Daisuke, Yoshizawa Kazuya, Yagi Makoto, Nishise Yuko, Ueno Yoshiyuki
Department of Gastroenterology, Yamagata University Faculty of Medicine, Yamagata, Japan.
Ther Apher Dial. 2014 Jun;18(3):252-7. doi: 10.1111/1744-9987.12212.
Tumor necrosis factor-α, (TNF)-α, a proinflammatory cytokine, is produced by activated granulocytes and monocytes (GMs) and implicated as a major factor in inflammatory bowel disease (IBD) pathogenesis. Reduction of TNF-α should improve IBD pathology. GM adsorptive apheresis (GMA) is an effective therapy for inflammatory disorders including IBD. GM adsorption to cellulose acetate (CA) beads induces anti-inflammatory cytokine release, although these effects on TNF-α release are not clarified. We hypothesized that GMA may inhibit TNF-α release. The aim of the present study was to clarify the effects of GM adsorption to CA beads on TNF-α release in vitro. Peripheral blood was incubated with and without CA beads and TNF-α was measured. For comparison, TNF-α was measured in another lipopolysaccharide (LPS)-containing peripheral blood sample incubated similarly. The amount of TNF-α in blood samples incubated with CA beads was significantly higher than in those incubated without beads, although it was significantly lower than TNF-α incubated with LPS-containing sample without beads. The amount of TNF-α after incubation with CA beads positively correlated with GM adsorption ratio. GM adsorption to CA beads induced a small amount of TNF-α release. This is the first report on TNF-α release induced via GM adsorption stimuli. The biological effects of TNF-α release during GM adsorption need to be clarified.
肿瘤坏死因子-α（TNF-α）是一种促炎细胞因子，由活化的粒细胞和单核细胞（GMs）产生，被认为是炎症性肠病（IBD）发病机制中的一个主要因素。降低TNF-α水平应能改善IBD的病理状况。GM吸附性血液成分分离术（GMA）是治疗包括IBD在内的炎症性疾病的一种有效疗法。GM吸附到醋酸纤维素（CA）珠上可诱导抗炎细胞因子释放，不过这些对TNF-α释放的影响尚不清楚。我们推测GMA可能抑制TNF-α释放。本研究的目的是阐明GM吸附到CA珠上对体外TNF-α释放的影响。将外周血分别与CA珠一起孵育和不与CA珠孵育，然后检测TNF-α水平。为作比较，在另一个同样孵育的含脂多糖（LPS）的外周血样本中检测TNF-α。与CA珠一起孵育的血样中TNF-α的量显著高于未与珠一起孵育的血样，尽管它显著低于未与珠一起孵育的含LPS样本中的TNF-α。与CA珠孵育后TNF-α的量与GM吸附率呈正相关。GM吸附到CA珠上诱导了少量TNF-α释放。这是关于通过GM吸附刺激诱导TNF-α释放的首篇报道。GM吸附过程中TNF-α释放的生物学效应有待阐明。
