Nishise Shoichi, Takeda Yuji, Nara Hidetoshi, Abe Yasuhiko, Sasaki Yu, Asao Hironobu, Ueno Yoshiyuki
Department of Gastroenterology, Yamagata University Faculty of Medicine, Yamagata, Japan.
Department of Immunology, Yamagata University Faculty of Medicine, Yamagata, Japan.
Ther Apher Dial. 2018 Jun;22(3):261-265. doi: 10.1111/1744-9987.12693. Epub 2018 May 9.
Circulating levels of soluble intercellular adhesion molecule-1 (sICAM-1) and vascular adhesion molecule-1 (sVCAM-1) are elevated in patients with inflammatory bowel disease. Cellulose acetate (CA) beads are used as carriers for granulocyte and monocyte (GM) adsorptive apheresis (GMA). We investigated the effect of CA beads on sICAM-1 and sVCAM-1 plasma concentrations in vitro. Because GM adsorption to CA beads increased with a rise in the incubation temperature in our previous study, peripheral blood was incubated with and without CA beads at 5, 25, 37, or 43 °C and plasma sICAM-1 and sVCAM-1 was measured. The sICAM-1 and sVCAM-1 concentrations in samples incubated with CA beads were significantly lower than those without CA beads at all four temperatures. However, no significant differences were observed both sICAM-1 and sVCAM-1 plasma levels at the four different temperatures after incubation with CA beads. These results suggest that independent of incubation temperature, sICAM-1 and sVCAM-1 are likely to adsorb CA beads. These molecules may be a new index for predicting the therapeutic effects of GMA.
炎症性肠病患者循环中的可溶性细胞间黏附分子-1（sICAM-1）和血管细胞黏附分子-1（sVCAM-1）水平升高。醋酸纤维素（CA）珠用作粒细胞和单核细胞（GM）吸附性血液成分分离术（GMA）的载体。我们在体外研究了CA珠对sICAM-1和sVCAM-1血浆浓度的影响。由于在我们之前的研究中GM对CA珠的吸附随着孵育温度的升高而增加，因此将外周血在5、25、37或43°C下分别在有和没有CA珠的情况下进行孵育，并测量血浆中的sICAM-1和sVCAM-1。在所有四个温度下，与CA珠一起孵育的样品中的sICAM-1和sVCAM-1浓度均显著低于没有CA珠的样品。然而，在与CA珠孵育后的四个不同温度下，sICAM-1和sVCAM-1的血浆水平均未观察到显著差异。这些结果表明，与孵育温度无关，sICAM-1和sVCAM-1可能会吸附在CA珠上。这些分子可能是预测GMA治疗效果的新指标。
