Luo Hai-qing, Xu Meng, Zhong Wang-tao, Cui Zhong-yi, Liu Fu-mei, Zhou Ke-yan, Li Xiang-yong
Center of Oncology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, China.
J BUON. 2014 Apr-Jun;19(2):435-9.
To investigate the effects of epigallocatechin-3-gallate (EGCG) on the expression of HIF-1α and vascular endothelial growth factor (VEGF) and cell growth in MCF-7 breast cancer cells.
MCF-7 human breast cancer cells were pretreated with different concentrations of EGCG (25, 50, 100 mg/L) for 48 h. The growth and proliferation of cells were analyzed by trypan blue staining in the pretreated MCF-7 cells. Furthermore, mRNA expression of HIF-1α and VEGF was detected by reverse transcriptase polymerase chain reaction (RT-PCR) analysis in the pretreated MCF-7 cells. Protein expression of HIF-1α was detected by Western blot, and the secreted protein level of VEGF in the supernatant of the culture medium was analyzed by enzyme linked immuno- sorbent assay (ELISA) in the MCF-7 cells pretreated with different concentrations of EGCG.
Cell growth decreased dramatically in MCF-7 cells treated with different concentrations of EGCG, compared with untreated (control) cells. Moreover, protein expression of HIF-1α and VEGF declined in a dose-dependent manner in MCF-7 cells pretreated with increasing concentrations of EGCG.
EGCG inhibits cell growth and proliferation of MCF-7 breast cancer cells, possibly by inhibiting the protein expression of HIF-1α and VEGF.
研究表没食子儿茶素-3-没食子酸酯（EGCG）对MCF-7乳腺癌细胞中缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）表达以及细胞生长的影响。
用不同浓度的EGCG（25、50、100mg/L）预处理MCF-7人乳腺癌细胞48小时。通过台盼蓝染色分析预处理后MCF-7细胞的生长和增殖情况。此外，通过逆转录聚合酶链反应（RT-PCR）分析检测预处理后MCF-7细胞中HIF-1α和VEGF的mRNA表达。通过蛋白质印迹法检测HIF-1α的蛋白质表达，并通过酶联免疫吸附测定（ELISA）分析不同浓度EGCG预处理的MCF-7细胞培养基上清液中VEGF的分泌蛋白水平。
与未处理（对照）细胞相比，用不同浓度EGCG处理的MCF-7细胞的生长显著降低。此外，在浓度递增的EGCG预处理的MCF-7细胞中，HIF-1α和VEGF的蛋白质表达呈剂量依赖性下降。
EGCG可能通过抑制HIF-1α和VEGF的蛋白质表达来抑制MCF-7乳腺癌细胞的生长和增殖。
