外泌体/葡萄球菌肠毒素B，一种抗胰腺癌的抗肿瘤化合物。

Exosome/staphylococcal enterotoxin B, an anti tumor compound against pancreatic cancer.

作者信息

Mahmoodzadeh Hosseini Hamideh, Ali Imani Fooladi Abbas, Soleimanirad Jafar, Reza Nourani Mohammad, Mahdavi Mehdi

机构信息

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

J BUON. 2014 Apr-Jun;19(2):440-8.

PMID:24965404

Abstract

PURPOSE

Exosomes (EXOs) are acellular vehicles used for cancer immunotherapy due to their immune-inducing properties. We synthesized a novel structure based on EXOs and staphylococcal enterotoxin B (SEB) and surveyed its cytostatic effect on a pancreatic cell line.

METHODS

EXOs were purified from tumor cells and SEB was anchored on it by protein transfer method. To determine the cytotoxic and apoptosis-inducing effect of this structure, treated cells with different concentrations of EXO/SEB were examined by MTT assay and Hoechst staining method. In addition, the expression rate of bcl-2, bax, bak, fas, bcl-xl and the activity of caspase-3 and caspase-9 were assessed.

RESULTS

We observed that 0.5 and 2.5 μg/100μl of EXO/ SEB significantly (p<0.001) stimulated apoptosis after 24 hrs. The concentrations of 0.5 and 2.5μg/100μl of EXO/SEB raised the expression rate of bax, bak, fas (p<0.001) but had no impact on bcl-2 and bcl-xl after 48 hrs. Furthermore, it was shown that 0.5, 2.5 and 5 μg/100μl of EXO/SEB only increased the activity of caspase-3 after 48 hrs (p<0.001).

CONCLUSION

Our designed structure, the EXO/SEB, is a novel model being able to induce apoptosis.

摘要

目的

外泌体（EXOs）因其免疫诱导特性而被用作癌症免疫治疗的无细胞载体。我们基于外泌体和葡萄球菌肠毒素B（SEB）合成了一种新型结构，并研究了其对胰腺细胞系的细胞抑制作用。

方法

从肿瘤细胞中纯化外泌体，并通过蛋白质转移方法将SEB锚定在其上。为了确定这种结构的细胞毒性和诱导凋亡作用，通过MTT法和Hoechst染色法检测用不同浓度的EXO/SEB处理的细胞。此外，评估bcl-2、bax、bak、fas、bcl-xl的表达率以及caspase-3和caspase-9的活性。

结果

我们观察到，0.5和2.5μg/100μl的EXO/SEB在24小时后显著（p<0.001）刺激细胞凋亡。0.5和2.5μg/100μl的EXO/SEB浓度在48小时后提高了bax、bak、fas的表达率（p<0.001），但对bcl-2和bcl-xl没有影响。此外，结果显示，0.5、2.5和5μg/100μl的EXO/SEB仅在48小时后增加了caspase-3的活性（p<0.001）。