Weissenbacher-Lang Christiane, Nedorost Nora, Weissenböck Herbert
Institute of Pathology and Forensic Veterinary Medicine, University of Veterinary Medicine, Veterinärplatz 1, 1210, Vienna, Austria.
J Eukaryot Microbiol. 2014 Sep-Oct;61(5):537-55. doi: 10.1111/jeu.12132. Epub 2014 Jul 24.
Pneumocystis sequences can be downloaded from GenBank for purposes as primer/probe design or phylogenetic studies. Due to changes in nomenclature and assignment, available sequences are presented with a variety of inhomogeneous information, which renders practical utilization difficult. The aim of this study was the descriptive evaluation of different parameters of 532 Pneumocystis sequences of mitochondrial and ribosomal origin downloaded from GenBank with regard to completeness and information content. Pneumocystis sequences were characterized by up to four different names. Official changes in nomenclature have only been partly implemented and the usage of the "forma specialis", a special feature of Pneumocystis, has only been established fragmentary in the database. Hints for a mitochondrial or ribosomal genomic origin could be found, but can easily be overlooked, which renders the download of wrong reference material possible. The specification of the host was either not available or variable regarding the used language and the localization of this information in the title or several subtitles, which limits their applicability in phylogenetic studies. Declaration of products and geographic origin was incomplete. The print version of this manuscript is completed by an online database which contains detailed information to every accession number included in the meta-analysis.
肺孢子菌序列可从GenBank下载，用于引物/探针设计或系统发育研究。由于命名法和分类的变化，现有的序列呈现出各种不均匀的信息，这使得实际应用变得困难。本研究的目的是对从GenBank下载的532个线粒体和核糖体来源的肺孢子菌序列的不同参数进行描述性评估，评估其完整性和信息含量。肺孢子菌序列最多有四个不同的名称。命名法的官方更改仅部分得到实施，“特殊形式”（肺孢子菌的一个特征）在数据库中的使用也只是零星建立。可以找到线粒体或核糖体基因组来源的提示，但很容易被忽略，这使得下载错误的参考材料成为可能。宿主的具体信息要么不可用，要么在所用语言以及该信息在标题或几个副标题中的位置方面存在差异，这限制了它们在系统发育研究中的适用性。产品和地理来源的声明不完整。本手稿的印刷版本由一个在线数据库补充，该数据库包含荟萃分析中每个登录号的详细信息。
