Khan Jawaid A
Plant Virus Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi-110025, India.
Bioinformation. 2014 May 20;10(5):251-5. doi: 10.6026/97320630010251. eCollection 2014.
Cotton leaf curl Allahabad virus (CLCuAV) belongs to genus Begomovirus, family Geminiviridae. It has single stranded monopartite DNA genome transmitted by whitefly (Bemisia tabaci). MicroRNAs (miRNAs) belong to class of endogeneous small RNAs which suppress expression of genes following cleavage or translational inhibition of target messenger RNAs. They are demonstrated to be involved in a number of plant processes such as, development, biotic and abiotic stresses. Employing in silico approach, high scoring miRNA-target pairs satisfying rules of minimum free energy and maximum complementarity were selected to investigate if they possess the potential to bind the genome CLCuAV. Our results revealed that miRNA species viz., ghr-miR2950 can target all the viral genes, ghr-miR408 targets overlapping transcripts of AC1 and AC2 genes; while ghr-miR394 and ghr-miR395a and miR395d could bind overlapping transcripts of AC1 and AC4 genes. This is the first report of prediction of cotton miRNAs which have the potential to target CLCuAV genes including AC1 and AC4, involved in viral replication and gene silencing suppression, respectively.
棉花曲叶阿拉哈巴德病毒（CLCuAV）属于双生病毒科菜豆金色花叶病毒属。它具有由烟粉虱（Bemisia tabaci）传播的单链单分体DNA基因组。微小RNA（miRNA）属于内源性小RNA类别，通过切割或抑制靶信使RNA的翻译来抑制基因表达。它们被证明参与许多植物过程，如发育、生物和非生物胁迫。采用计算机模拟方法，选择了满足最小自由能和最大互补性规则的高得分miRNA-靶标对，以研究它们是否具有结合CLCuAV基因组的潜力。我们的结果表明，miRNA种类，即ghr-miR2950可以靶向所有病毒基因，ghr-miR408靶向AC1和AC2基因的重叠转录本；而ghr-miR394、ghr-miR395a和miR395d可以结合AC1和AC4基因的重叠转录本。这是关于预测棉花miRNA的首次报道，这些miRNA有可能靶向CLCuAV基因，包括分别参与病毒复制和基因沉默抑制的AC1和AC4基因。
