Akhter Yusuf, Khan Jawaid Ahmad
Plant Virus Laboratory, Department of Biosciences, Jamia Millia Islamia (Central University), New Delhi 110025, India.
Centre for Computational Biology and Bioinformatics, School of Life Sciences, Central University of Himachal Pradesh, India.
Gene. 2018 Jan 5;638:60-65. doi: 10.1016/j.gene.2017.09.061. Epub 2017 Sep 28.
Cotton leaf curl Burewala virus (CLCuBV, genus Begomovirus) causes devastating cotton leaf curl disease. Among various known virus controlling strategies, RNAi-mediated one has shown potential to protect host crop plants. Micro(mi) RNAs, are the endogenous small RNAs and play a key role in plant development and stress resistance. In the present study we have identified cotton (Gossypium hirsutum)-encoded miRNAs targeting the CLCuBV. Based on threshold free energy and maximum complementarity scores of host miRNA-viral mRNA target pairs, a number of potential miRNAs were annotated. Among them, ghr-miR168 was selected as the most potent candidate, capable of targeting several vital genes namely C1, C3, C4, V1 and V2 of CLCuBV genome. In addition, ghr-miR395a and ghr-miR395d were observed to target the overlapping transcripts of C1 and C4 genes. We have verified the efficacy of these miRNA targets against CLCuBV following suppression of RNAi-mediated virus control through translational inhibition or cleavage of viral mRNA.
棉花卷叶布勒瓦拉病毒（CLCuBV，菜豆金色花叶病毒属）引发毁灭性的棉花卷叶病。在各种已知的病毒控制策略中，RNA干扰介导的策略已显示出保护寄主作物的潜力。微小（mi）RNA是内源性小RNA，在植物发育和抗逆性中起关键作用。在本研究中，我们鉴定了靶向CLCuBV的棉花（陆地棉）编码的miRNA。基于寄主miRNA-病毒mRNA靶标对的无阈值自由能和最大互补性得分，注释了许多潜在的miRNA。其中，ghr-miR168被选为最有效的候选物，能够靶向CLCuBV基因组的几个重要基因，即C1、C3、C4、V1和V2。此外，观察到ghr-miR395a和ghr-miR395d靶向C1和C4基因的重叠转录本。通过翻译抑制或切割病毒mRNA抑制RNA干扰介导的病毒控制后，我们验证了这些miRNA靶标对CLCuBV的有效性。
