Cobanoglu Nevin, Ozer Fusun, Demirci Mustafa, Erganis Osman, Imazato Satoshi
Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, Konya, Turkiye.
Department of Preventive and Restorative Sciences, University of Pennsylvania, Philadelphia, PA, USA.
Eur J Dent. 2014 Apr;8(2):166-171. doi: 10.4103/1305-7456.130590.
The aim of this study was to investigate the effects of two bonding systems, with and without antibacterial monomers, on marginal bacterial and dye leakage.
Class V cavities were prepared in extracted teeth for a bacterial leakage test, and the teeth were sterilized using a steam autoclave. Four cavities were not restored for the controls, and the other teeth were divided into two groups (n = 16 cavities each): Clearfil Protect Bond group (CPB) and Clearfil SE Bond group (CSE). After application of the bonding agent, the cavities were restored using a composite resin (Clearfil AP-X). The teeth were thermocycled, stored in a broth culture of 1.56 × 108 colony forming units (CFU)/ml of Streptococcus mutans at 37°C for 10 days, and subsequently processed for bacterial staining. Sections from the demineralized teeth were evaluated under a light microscope. In the dye leakage test, the cavities were restored as described in the bacterial penetration test. After thermocycling, the teeth were immersed in 5% basic fuchsin for 24 h, and then divided in half and observed under a stereomicroscope. The data were analyzed using the Kruskal-Wallis and Mann-Whitney U-tests (P = 0.05).
The bacterial stain was detected at the cavity wall of five cavities in both bonding systems. Additionally, two cavities in the CSE group, one cavity in the CPB group, and all control cavities showed bacterial staining within the cut dentinal tubules. Dye staining at the axial cavity wall was detected in only three of the teeth for both bonding systems.
The bonding systems used in this study provided an acceptable marginal seal to prevent bacterial and dye leakage.
本研究旨在调查含与不含抗菌单体的两种粘结系统对边缘细菌渗漏和染料渗漏的影响。
在拔除的牙齿上制备V类洞进行细菌渗漏测试，并用蒸汽高压灭菌器对牙齿进行消毒。四个洞不做修复作为对照，其他牙齿分为两组（每组16个洞）：Clearfil Protect Bond组（CPB）和Clearfil SE Bond组（CSE）。应用粘结剂后，用复合树脂（Clearfil AP-X）修复洞。对牙齿进行热循环处理，在37℃下于每毫升含1.56×10⁸ 菌落形成单位（CFU）的变形链球菌肉汤培养物中储存10天，随后进行细菌染色处理。在光学显微镜下评估脱矿牙齿的切片。在染料渗漏测试中，按照细菌渗透测试中的描述修复洞。热循环处理后，将牙齿浸入5%碱性品红中24小时，然后切成两半，在体视显微镜下观察。使用Kruskal-Wallis检验和Mann-Whitney U检验分析数据（P = 0.05）。
在两种粘结系统的五个洞的洞壁处均检测到细菌染色。此外，CSE组的两个洞、CPB组的一个洞以及所有对照洞在切割的牙本质小管内均显示细菌染色。两种粘结系统仅在三颗牙齿的轴向洞壁处检测到染料染色。
本研究中使用的粘结系统提供了可接受的边缘封闭，以防止细菌和染料渗漏。
