Guo Luyan, Chen Shuqin, Jiang Hongye, Huang Jiaming, Jin Wenyan, Yao Shuzhong
Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, China.
Int J Clin Exp Pathol. 2014 Apr 15;7(5):2102-12. eCollection 2014.
There is increasing evidence suggesting that S100P has a significant role in cancer, and is associated with poor clinical outcomes. The expression of S100P mRNA and protein in endometrial cancer and normal endometrium tissues was detected by real-time quantitative RT-PCR and immunohistochemistry. Moreover, we reduced the expression of S100P in HEC-1A and Ishikawa endometrial cancer cell lines by siRNA transfection. Based on the reduced S100P mRNA expression, we measured the effects of S100P on cellular proliferation by the cell-counting kit-8. Nuclear β-catenin protein level was detected by western blotting. Cyclin D1 and c-myc mRNA expression regulated by β-catenin was detected by real-time quantitative RT-PCR. We found that the expression of S100P mRNA and protein increased in endometrial cancer tissues compared with the normal endometrium. Local S100P expression progressively increased from pathologic differenciation grade 1 to 3. After reducing the S100P expression, the cellular proliferation ability, nuclear β-catenin protein level, cyclin D1 and c-myc mRNA levels reduced. It indicated that S100P could promote cell proliferation by increasing nuclear translocation of β-catenin. The expression of S100P mRNA and protein in endometrial cancer significantly increased and is associated with pathologic differenciation grade. S100P may promote endometrial cell proliferation by increasing nuclear translocation of β-catenin.
越来越多的证据表明，S100P在癌症中发挥着重要作用，且与不良临床结果相关。通过实时定量逆转录聚合酶链反应（RT-PCR）和免疫组织化学检测子宫内膜癌组织和正常子宫内膜组织中S100P mRNA和蛋白的表达。此外，我们通过小干扰RNA（siRNA）转染降低了人子宫内膜癌细胞系HEC-1A和Ishikawa中S100P的表达。基于S100P mRNA表达的降低，我们使用细胞计数试剂盒-8（CCK-8）测量S100P对细胞增殖的影响。通过蛋白质印迹法检测细胞核β-连环蛋白的蛋白水平。通过实时定量RT-PCR检测由β-连环蛋白调节的细胞周期蛋白D1（Cyclin D1）和原癌基因c-myc的mRNA表达。我们发现，与正常子宫内膜相比，子宫内膜癌组织中S100P mRNA和蛋白的表达增加。局部S100P表达从病理分化1级到3级逐渐增加。降低S100P表达后，细胞增殖能力、细胞核β-连环蛋白蛋白水平、Cyclin D1和c-myc mRNA水平降低。这表明S100P可通过增加β-连环蛋白的核转位来促进细胞增殖。子宫内膜癌中S100P mRNA和蛋白的表达显著增加，且与病理分化程度相关。S100P可能通过增加β-连环蛋白的核转位来促进子宫内膜细胞增殖。
