氯高铁血红素通过诱导大鼠局灶性脑缺氧缺血中的外源性脑红蛋白发挥神经保护作用。
Hemin offers neuroprotection through inducing exogenous neuroglobin in focal cerebral hypoxic-ischemia in rats.
作者信息
Song Xue, Xu Rui, Xie Fei, Zhu Haiyuan, Zhu Ji, Wang Xin
机构信息
Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University Chongqing, 400016, China.
Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School Boston, Massachusetts 02115, USA.
出版信息
Int J Clin Exp Pathol. 2014 Apr 15;7(5):2163-71. eCollection 2014.
Abstract
OBJECTIVE
To investigate the inducible effect of hemin on exogenous neuroglobin (Ngb) in focal cerebral hypoxic-ischemia in rats.
METHODS
125 healthy SD rats were randomly divided into five groups: sham-operation control group, operation group, hemin treatment group, exogenous Ngb treatment group, and hemin and exogenous Ngb joint treatment group. Twenty-four hours after focal cerebral hypoxic-ischemia, Ngb expression was evaluated by immunocytochemistry, RT-PCR, and western blot analyses, while the brain water content and infarct volume were examined.
RESULTS
Immunocytochemistry, RT-PCR, and western blot analyses showed more pronounced Ngb expression in the hemin and exogenous Ngb joint operation group than in the hemin or exogenous Ngb individual treatment groups, thus producing significant differences in brain water content and infarct volume (p<0.05).
CONCLUSIONS
Hemin may be beneficial in protecting against focal cerebral hypoxic-ischemia through inducing the expression of exogenous Ngb.
摘要
目的
探讨氯化血红素对大鼠局灶性脑缺氧缺血中外源性神经球蛋白（Ngb）的诱导作用。
方法
将125只健康SD大鼠随机分为五组：假手术对照组、手术组、氯化血红素治疗组、外源性Ngb治疗组、氯化血红素与外源性Ngb联合治疗组。局灶性脑缺氧缺血24小时后，通过免疫细胞化学、RT-PCR和蛋白质免疫印迹分析评估Ngb表达，同时检测脑含水量和梗死体积。
结果
免疫细胞化学、RT-PCR和蛋白质免疫印迹分析显示，氯化血红素与外源性Ngb联合手术组的Ngb表达比氯化血红素或外源性Ngb单独治疗组更明显，从而在脑含水量和梗死体积方面产生显著差异（p<0.05）。
结论
氯化血红素可能通过诱导外源性Ngb的表达，对局灶性脑缺氧缺血起到保护作用。
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