非病毒质粒递送碱性成纤维细胞生长因子和低强度脉冲超声对下颌髁突生长的影响：一项初步研究。
Effect of nonviral plasmid delivered basic fibroblast growth factor and low intensity pulsed ultrasound on mandibular condylar growth: a preliminary study.
作者信息
Kaur Harmanpreet, Uludağ Hasan, El-Bialy Tarek
机构信息
Department of Dentistry, Faculty of Medicine and Dentistry, Medical Science Graduate Program, University of Alberta, Edmonton, AB, Canada T6G 2E1.
Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB, Canada T6G 2E1.
出版信息
Biomed Res Int. 2014;2014:426710. doi: 10.1155/2014/426710. Epub 2014 May 20.
OBJECTIVE
Basic fibroblast growth factor (bFGF) is an important regulator of tissue growth. Previous studies have shown that low intensity pulsed ultrasound (LIPUS) stimulates bone growth. The objective of this study was to evaluate the possible synergetic effect of LIPUS and local injection of nonviral bFGF plasmid DNA (pDNA) on mandibular growth in rats.
DESIGN
Groups were control, blank pDNA, bFGF pDNA, LIPUS, and bFGF pDNA + LIPUS. Treatments were performed for 28 days. Significant increase was observed in mandibular height and condylar length in LIPUS groups. MicroCT analysis showed significant increase in bone volume fraction in bFGF pDNA + LIPUS group. Histomorphometric analysis showed increased cell count and condylar proliferative and hypertrophic layers widths in bFGF pDNA group.
RESULTS
Current study showed increased mandibular condylar growth in either bFGF pDNA or LIPUS groups compared to the combined group that showed only increased bone volume fraction.
CONCLUSION
It appears that there is an additive effect of bFGF + LIPUS on the mandibular growth.
目的
碱性成纤维细胞生长因子（bFGF）是组织生长的重要调节因子。先前的研究表明，低强度脉冲超声（LIPUS）可刺激骨骼生长。本研究的目的是评估LIPUS与局部注射非病毒bFGF质粒DNA（pDNA）对大鼠下颌骨生长的可能协同作用。
设计
分为对照组、空白pDNA组、bFGF pDNA组、LIPUS组和bFGF pDNA + LIPUS组。治疗持续28天。在LIPUS组中观察到下颌高度和髁突长度显著增加。MicroCT分析显示bFGF pDNA + LIPUS组的骨体积分数显著增加。组织形态计量学分析显示bFGF pDNA组的细胞计数增加，髁突增殖层和肥大层宽度增加。
结果
当前研究表明，与仅显示骨体积分数增加的联合组相比，bFGF pDNA组或LIPUS组的下颌髁突生长均增加。
结论
bFGF + LIPUS对下颌骨生长似乎具有相加作用。
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