de Boer M W, Zheng T, Buddle B M, McDougall S
a Anexa Animal Health , Cognosco , PO Box 21, Morrinsville , 3300 , New Zealand.
N Z Vet J. 2014 Nov;62(6):351-5. doi: 10.1080/00480169.2014.933683. Epub 2014 Sep 29.
To detect the presence of bovine herpesvirus (BoHV) type 4 in New Zealand dairy cows with clinical metritis.
Serum samples taken from 92 dairy cows with clinical metritis, each from a different farm, were tested for the presence of antibodies against BoHV-4 using a commercially available, indirect ELISA. Peripheral blood mononuclear cells (PBMC) were collected from 10 BoHV-4 seropositive cows, and PBMC were examined by a pan-herpesvirus nested PCR to detect herpesvirus. PCR products were sequenced directly and a proportion of the PCR products were cloned and sequenced to identify the virus present.
Antibodies to BoHV-4 were detected in 23/92 (25%) serum samples. The pan-herpesvirus PCR was positive in 8/10 PBMC samples. Cloning and sequencing identified that all of the eight PCR-positive PBMC contained bovine lymphotropic herpesvirus (BLHV); no BoHV-4 DNA was detected.
This study reports the finding of the presence of apparent antibodies to BoHV-4, and BLHV DNA in New Zealand dairy cows affected by metritis.
Bovine herpesvirus type 4 and BLHV are reported to have the potential to cause reproduction failure in cows. This is the first report of apparent BoHV-4 antibodies, and BLHV in New Zealand. The importance and epidemiology of these viruses in cattle in New Zealand requires further investigation.
检测患有临床型子宫炎的新西兰奶牛中牛疱疹病毒4型（BoHV-4）的存在情况。
从92头患有临床型子宫炎的奶牛（每头来自不同农场）采集血清样本，使用市售间接ELISA检测抗BoHV-4抗体的存在情况。从10头BoHV-4血清阳性奶牛中采集外周血单个核细胞（PBMC），通过泛疱疹病毒巢式PCR检测疱疹病毒。对PCR产物直接测序，并对部分PCR产物进行克隆和测序以鉴定存在的病毒。
在23/92（25%）的血清样本中检测到抗BoHV-4抗体。泛疱疹病毒PCR在8/10的PBMC样本中呈阳性。克隆和测序鉴定出所有8个PCR阳性的PBMC均含有牛嗜淋巴细胞疱疹病毒（BLHV）；未检测到BoHV-4 DNA。
本研究报告了在患有子宫炎的新西兰奶牛中发现抗BoHV-4的明显抗体以及BLHV DNA的情况。
据报道，牛疱疹病毒4型和BLHV有可能导致奶牛繁殖失败。这是新西兰首次报告出现明显的BoHV-4抗体以及BLHV。这些病毒在新西兰牛群中的重要性和流行病学需要进一步研究。
